Food allergens
Reliable analytical solutions for your allergen management
Food allergen testing
The importance of food allergen management
For most people, consuming food is harmless, but for those with food allergies, it can cause life-threatening reactions and significantly impact their quality of life. Effective food allergen management using reliable testing methods is therefore critical.
Prevalence of food allergies and intolerances
Scientists estimate that approximately 2-4% of adults and 6-8% of children suffer from food allergies. Additionally, a significant number of people have food intolerances, although their exact prevalence is difficult to determine due to a lack of comprehensive studies. Unlike food intolerances, food allergies are highly dangerous because they are caused by immune system reactions triggered by proteins to which individuals are sensitized.
The need for accurate allergen labeling
Labeling allergenic proteins, known as allergens, is crucial for protecting allergic individuals. Hidden allergens, which can appear in convenience foods or as unintended contaminants during storage and production, pose significant risks.
Allergen regulation
To safeguard allergic individuals, the EU implemented food labeling regulations, which are continuously updated (EU Regulation 1169/2011, effective December 2014). Since November 2005, food producers are required to label ingredients that can cause allergies or intolerances. This includes a comprehensive list of substances and products derived from them.
Furthermore, sulfur dioxide and sulfites in concentrations over 10 milligrams per kilogram must be labeled. Other countries have similar regulations tailored to country-specific allergens, such as Histamine.
An interactive map of international allergen regulations is available from the Food Allergy Research and Resource Program (FARRP).
Allergen risk management
In most production lines, multiple products are produced, leading to potential unintended allergen contamination. This can occur through cross-contact of raw materials, inadequate storage, shared production equipment, airborne dust, improper reuse of leftovers, or faulty packaging. To protect consumers and ensure accurate labeling, an HACCP (Hazard Analysis Critical Control Point) concept, including reliable on-site allergen analysis, must be implemented. Effective measures such as strategic production schedules and rigorous cleaning practices can minimize cross-contact with allergens.
Test kits for allergen analysis
R-Biopharm offers a range of immunochemical (ELISA, lateral flow tests) and molecular biological (PCR) techniques for allergen analysis. Our extensive portfolio includes test kits for almond, casein, crustacean, egg, gluten/gliadin, hazelnut, β-lactoglobulin, lupine, milk, mustard, peanut, sesame, and soy. Conducting allergen analysis requires an allergen-free laboratory to ensure accurate results.
The bioavid lateral flow tests are now available in a new format, which includes a hook line and everything needed for cleaning control. Find out more about our bioavid lateral flow tests.
Explore our video section to get hands-on information on allergen analysis and the test procedure of our kits.
Product portfolio
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| RIDASCREEN®EASY Hazelnut |
RIDASCREEN®EASY Hazelnut (Art. No. RAE6401) is a sandwich enzyme immunoassay for the quantitative analysis of contaminations by hazelnut protein in foods. Hygiene samples can be investigated according to an application note, too. Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) |
RAE6401 |
| RIDASCREEN®EASY Crustacean |
RIDASCREEN®EASY Crustacean (Art. No. RAE3001) is a sandwich enzyme immunoassay for the quantitative analysis of contaminations by crustacean protein in foods. Hygiene samples can be investigated according to an application note, too. Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | RAE3001 |
| RIDASCREEN® Walnut |
RIDASCREEN® Walnut (Art. No. R6601) is a sandwich enzyme immunoassay for the quantitative analysis of raw and roasted walnut or walnut protein in food. Due to the large number of different food products, the following samples were examined as … Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R6601 |
| RIDASCREEN®EASY Mustard |
RIDASCREEN®EASY Mustard is an enzyme immunoassay for the quantitative determination of mustard. Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | RAE8201 |
| RIDASCREEN®EASY Gluten |
Fast and easy ELISA test method for gluten detection! Ensures a safe, fast and easy quantitative analysis of gluten residues from wheat, rye and barley within 30 minutes, delivering results that are in line with the Codex Alimentarius Type I Method … Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | RAE7071 |
| RIDASCREEN® Total Gluten |
Specialty ELISA test method for gluten detection in oats! Ensures safe quantitative analysis of gluten residues from gluten-containing cereals (wheat, rye and barley) in oat and oat products. RIDASCREEN® Total Gluten is a sandwich enzyme immunoassay … Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R7041 |
| RIDASCREEN® Peanut |
RIDASCREEN® Peanut is a sandwich enzyme immunoassay developed for the quantitative analysis of peanut or peanut proteins in food. Due to the large number of different food products, the following samples were examined representatively for different … Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R6811 |
| RIDASCREEN® Egg |
RIDASCREEN® Egg is a sandwich enzyme immunoassay developed for the quantitative analysis of native and processed hen´s egg in foods. The following foods were examined as representatives of the group of products including baked goods, sweets, … Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R6411 |
| RIDASCREEN®FAST Gliadin sensitive |
Fast and sensitive ELISA test method for gluten detection Ensures a safe, fast and sensitive quantitative analysis of gluten residues from gluten containing cereals (wheat, rye and barley). RIDASCREEN®FAST Gliadin sensitive is a R5-based sandwich … Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R7051 |
| RIDASCREEN® Gliadin |
Reference ELISA test method for gluten detection! Ensure safe quantitative analysis of prolamins from wheat (gliadin), rye (secalin) and barley (hordein) in food with the reference method. The RIDASCREEN® Gliadin in combination with the Cocktail … Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R7001 |
| RIDASCREEN®FAST Cashew |
The RIDASCREEN®FAST Cashew is a sandwich enzyme immunoassay developed for the quantitative analysis of Cashew in food. Exemplarily for the food groups pastries and sweets have been tested during the assay’s development: cookies, chocolate and ice … Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R6872 |
| RIDASCREEN®FAST Hazelnut |
RIDASCREEN®FAST Hazelnut is a sandwich enzyme immunoassay for the quantitative analysis of hazelnut (e.g. traces of hazelnut) in food such as cereals, bakery products, ice cream and chocolate. Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R6802 |
| RIDASCREEN®FAST Sesame |
RIDASCREEN®FAST Sesame is a sandwich enzyme immunoassay for the quantitative analysis of sesame or parts of sesame (as ingredient components or contamination) in instant soup, chocolate dessert, bakery products and crackers. Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R7202 |
| RIDASCREEN®FAST Soya |
RIDASCREEN®FAST Soya is a sandwich enzyme immunoassay for the quantitative analysis of native and processed soya proteins in food. Such products are beverages and food as sausages, dressings, bakery products, ice, chocolate, soup, sauce, margarine, … Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R7102 |
| RIDASCREEN®FAST Milk |
RIDASCREEN®FAST Milk is a sandwich enzyme immunoassay to quantify milk proteins (casein and ß-lactoglobulin) in food containing whey, milk or milk powder such as sausages, ice cream, chocolate, bakery goods, cake and bread mix, soups, sauces, … Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R4652 |
| RIDASCREEN®FAST β-Lactoglobulin |
RIDASCREEN®FAST β-Lactoglobulin is a sandwich enzyme mmunoassay for the quantitative analysis of native and processed β-lactoglobulin in rice crispies, chocolate and sausage. Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R4912 |
| RIDASCREEN® ß-Lactoglobulin |
RIDASCREEN® ß-Lactoglobulin is a competitive enzyme immunoassay for the quantitative analysis of β-lactoglobulin in hydrolyzed milk products including hypoallergenic baby food. The assay is calibrated to hydrolyzed β-lactoglobulin check … Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R4901 |
| RIDASCREEN®FAST Lysozym |
RIDASCREEN®FAST Lysozym is a sandwich enzyme immunoassay for the quantitative analysis of lysozyme (hen’s egg protein) in food like wine, cheese and sausage. Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R6452 |
| RIDASCREEN®FAST Lupine |
RIDASCREEN®FAST Lupine is a sandwich enzyme immunoassay for the quantitative analysis of lupine proteins in food (e.g. juices, wine, beer, sausages, noodles, chocolate, bakery products or ice cream). Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R6102 |
| RIDASCREEN® Gliadin competitive |
Specialty ELISA test method (competitive) for gluten detection in fermented and hydrolyzed food! Ensures safe quantitative analysis of prolamine peptide fragments from wheat (gliadin), rye (secalin) and barley (hordein) in fermented or hydrolysed … Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R7021 |
| RIDASCREEN®FAST Gliadin |
Fast ELISA test method for gluten detection! Ensures a safe and fast quantitative analysis of prolamins from wheat (gliadin), rye (secalin) and barley (hordein) in food. RIDASCREEN®FAST Gliadin is a sandwich enzyme immunoassay for the quantitative … Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R7002 |
| RIDASCREEN®FAST Almond |
RIDASCREEN®FAST Almond is a sandwich enzyme immunoassay for the quantitative analysis of almond or parts of almond in food. As representative samples for the food groups pastries and sweets the following matrices have been tested during the assay´s … Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R6901 |
| RIDASCREEN®FAST Egg Protein |
RIDASCREEN®FAST Egg Protein is a sandwich enzyme immunoassay for the quantitative analysis of whole egg (-powder) in food like salad dressings, sausages, wines, baking-mixtures for cakes or bread and ice cream. Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R6402 |
| RIDASCREEN®FAST Casein |
RIDASCREEN®FAST Casein is a sandwich enzyme immunoassay for the quantitative analysis of casein in food like bakery goods, cake and bread mix, non-hydrolyzed milk-based infant formula, ice cream, beverages, chocolate, wine and sausages. Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R4612 |
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| RIDASCREEN® Histamine (enzymatic) |
RIDASCREEN® Histamine (enzymatic) is used for the quantitative determination of histamine in fresh fish, canned fish, fish meal, wine and cheese. Read more |
Microtiterplate with 96 wells (12 strips with 8 removable wells each) | R1605 |
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| bioavid Lateral Flow Brazil Nut incl. Hook Line |
The Lateral Flow Brazil Nut (Art. No. BLH702-15), with included hook line from bioavid, is an immunochromatographic test for the sensitive and qualitative detection of brazil nut residues on surfaces (e.g. swab test for the hygiene control in food … Read more |
15 test strips (15 determinations) | BLH702-15 |
| RIDA®QUICK Gluten quant. |
Fastest and most simple quantitative LFD method for gluten detection! Ensures a safe, fast and simple quantitative analysis of gluten residues from wheat, rye and barley on surfaces, in clean-in-place (CIP) water and in foods (raw and processed) in … Read more |
15 test strips | RAL7073 |
| bioavid Lateral Flow Soy incl. Hook Line |
The Lateral Flow Soy (Art. No. BLH712-15), with included hook line from bioavid, is an immunochromatographic test for the sensitive and qualitative detection of raw and processed soy residues on surfaces (e.g. swab test for the hygiene control in … Read more |
15 test strips (15 determinations) | BLH712-15 |
| bioavid Lateral Flow Macadamia incl. Hook Line |
The Lateral Flow Macadamia (Art. No. BLH705-15), with included hook line from bioavid, is an immunochromatographic test for the sensitive and qualitative detection of macadamia residues on surfaces (e.g. swab test for the hygiene control in food … Read more |
15 test strips (15 determinations) | BLH705-15 |
| bioavid Lateral Flow Pistachio incl. Hook Line |
The Lateral Flow Pistachio (Art. No. BLH711-15), with included hook line from bioavid, is an immunochromatographic test for the sensitive and qualitative detection of pistachio residues on surfaces (e.g. swab test for the hygiene control in food … Read more |
15 test strips (15 determinations) | BLH711-15 |
| bioavid Lateral Flow Walnut incl. Hook Line |
The Lateral Flow Walnut (Art. No. BLH707-15), with included hook line from bioavid, is an immunochromatographic test for the sensitive and qualitative detection of walnut residues on surfaces (e.g. swab test for the hygiene control in food production … Read more |
15 test strips (15 determinations) | BLH707-15 |
| bioavid Lateral Flow Crustacean incl. Hook line |
The Lateral Flow Crustacean (Art. No. BLH716-15), with included hook line from bioavid, is an immunochromatographic test for the sensitive and qualitative detection of crustacean residues on surfaces (e.g. swab test for the hygiene control in food … Read more |
15 test strips (15 determinations) | BLH716-15 |
| bioavid Lateral Flow Casein incl. Hook line |
The Lateral Flow Casein (Milk) (Art. No. BLH714-15), with included hook line from bioavid, is an immunochromatographic test for the sensitive and qualitative detection of casein residues on surfaces (e.g. swab test for the hygiene control in food … Read more |
15 test strips (15 determinations) | BLH714-15 |
| bioavid Lateral Flow Cashew incl. Hook line |
The Lateral Flow Cashew (Art. No. BLH710-15), with included hook line from bioavid, is an immunochromatographic test for the sensitive and qualitative detection of cashew residues on surfaces (e.g. swab test for the hygiene control in food production … Read more |
15 test strips (15 determinations) | BLH710-15 |
| bioavid Lateral Flow Sesame incl. Hook line |
The Lateral Flow Sesame (Art. No. BLH709-15), with included hook line from bioavid, is an immunochromatographic test for the sensitive and qualitative detection of sesame residues on surfaces (e.g. swab test for the hygiene control in food production … Read more |
15 test strips (15 determinations) | BLH709-15 |
| bioavid Lateral Flow Egg incl. Hook line |
The Lateral Flow Egg (Art. No. BLH708-15), with included hook line from bioavid, is an immunochromatographic test for the sensitive and qualitative detection of egg residues on surfaces (e.g. swab test for the hygiene control in food production … Read more |
15 test strips (15 determinations) | BLH708-15 |
| bioavid Lateral Flow Peanut incl. Hook line |
The Lateral Flow Peanut (Art. No. BLH706-15), with included hook line from bioavid, is an immunochromatographic test for the sensitive and qualitative detection of peanut residues on surfaces (e.g. swab test for the hygiene control in food production … Read more |
15 test strips (15 determinations) | BLH706-15 |
| bioavid Lateral Flow Hazelnut incl. Hook line |
The Lateral Flow Hazelnut (Art. No. BLH704-15), with included hook line from bioavid, is an immunochromatographic test for the sensitive and qualitative detection of hazelnut residues on surfaces (e.g. swab test for the hygiene control in food … Read more |
15 test strips (15 determinations) | BLH704-15 |
| bioavid Lateral Flow Mustard incl. Hook line |
The Lateral Flow Mustard (Art. No. BLH703-15), with included hook line from bioavid, is an immunochromatographic test for the sensitive and qualitative detection of mustard residues on surfaces (e.g. swab test for the hygiene control in food … Read more |
15 test strips (15 determinations) | BLH703-15 |
| bioavid Lateral Flow Almond incl. Hook line |
The Lateral Flow Almond (Art. No. BLH701-15), with included hook line from bioavid, is an immunochromatographic test for the sensitive and qualitative detection of almond residues on surfaces (e.g. swab test for the hygiene control in food production … Read more |
15 test strips (15 determinations) | BLH701-15 |
| bioavid Lateral Flow Coconut incl. Hook line |
The Lateral Flow Coconut (Art. No. BLH700-15), with included hook line from bioavid, is an immunochromatographic test for the sensitive and qualitative detection of coconut residues on surfaces (e.g. swab test for the hygiene control in food … Read more |
15 test strips (15 determinations) | BLH700-15 |
| bioavid Lateral Flow Milk |
The Lateral Flow Milk (Art. No. BL623-15) from bioavid is an immunochromatographic test for the sensitive and qualitative detection of milk residues on surfaces (e.g. swab test for the hygiene control in food production lines), in cleaning / process … Read more |
15 test strips (15 determinations) | BL623-15 |
| RIDA®QUICK Soya |
RIDA®QUICK Soya is an immunochromatographic test for the qualitative detection of soya contamination (native and processed) on surfaces (swab test for the hygiene control in production and in laboratories) and in food (biscuit, margarine, chocolate, … Read more |
25 x test strips in reclosable tube for 25 determinations |
R7103 |
| RIDA®QUICK Gliadin |
Fast and simple qualitative LFD test method for the detection of gluten! Ensures safe, fast and simple qualitative analysis of gluten on surfaces, in clean-in-pace (CIP) water and food (raw and processed). RIDA®QUICK Gliadin is an R5-based … Read more |
25 x test strips | R7003 |
| RIDA®QUICK Gliadin (single packaged) |
Fast and simple qualitative LFD test method for the detection of gluten! Ensures safe, fast and simple qualitative analysis of gluten on surfaces, in clean-in-place (CIP) water and food (raw and processed). RIDA®QUICK Gliadin (single packaged) is … Read more |
25 x test strips (single packaged) |
R7004 |
| RIDA®QUICK Gliadin (ready to swab) |
Fast and simple qualitative LFD test method for the detection of gluten by direct swabbing of surfaces! Ensures safe, fast and simple qualitative analysis of gluten on surfaces and in clean-in-pace (CIP) waters. The kit variant RIDA®QUICK Gliadin … Read more |
25 x test strips (single packaged), 25 prefilled vials with ready-to-use sample buffer, for 25 determinations |
R7005 |
| bioavid Swabbing kit |
The bioavid Swabbing kit is used for the sampling of allergen residues on surfaces (e.g. in food production lines) for bioavid allergen rapid tests (lateral flow tests). Read more |
30 x sample tubes 30 x plastic swabs 30 x plastic pipettes |
BS803-30 |
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| SureFood® ALLERGEN 4plex SEAFOOD |
The SureFood® ALLERGEN 4plex SEAFOOD is a multiplex real-time PCR for the qualitative detection and differentiation of specific fish, crustaceans and molluscs DNA. Read more |
100 reactions | S3405 |
| SureFood® ALLERGEN Coconut |
The SureFood® ALLERGEN Coconut is a real-time PCR for the direct, qualitative detection of specific coconut (Cocos nucifera) DNA sequences in food. Read more |
100 reactions | S3621 |
| SureFood® ALLERGEN 4plex Almond/Pistachio/Cashew+IAC |
The SureFood® ALLERGEN 4plex Almond/Pistachio/Cashew+IAC is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific almond (Prunus dulcis), pistachio (Pistacia vera) and cashew (Anacardium occidentale) DNA … Read more |
100 reactions | S3406 |
| SureFood® ALLERGEN 4plex EU NUTS |
The SureFood® ALLERGEN 4plex EU NUTS is a multiplex real-time PCR for the direct, qualitative detection and differentiation of almond (Prunus dulcis), cashew (Anacardium occidentale), pistachio (Pistacia vera), peanut (Arachis hypogaea), hazelnut … Read more |
100 reactions | S3404 |
| SureFood® 4plex LEGUMES |
The SureFood® 4plex LEGUMES is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific DNA sequences of legumes (Fabaceae), bean (Phaseolus spp. and Vigna spp.) and pea (Pisum sativum). Read more |
100 reactions | S7008 |
| SureFood® ALLERGEN Shea Nut |
The SureFood® ALLERGEN Shea Nut is a real-time PCR for the direct, qualitative detection of specific shea nut (Vitellaria paradoxa) DNA sequences in raw material. Read more |
100 reactions | S3622 |
| SureFood® ALLERGEN Beechnut |
The SureFood® ALLERGEN Beechnut is a real-time PCR for the direct qualitative detection of specific beechnut (Fagus sylvatica) DNA sequences in food. Read more |
100 reactions | S3628 |
| SureFood® ALLERGEN Insects |
The SureFood® ALLERGEN Insects is a real-time PCR for the direct, qualitative detection of specific insects (Insecta) DNA sequences. Read more |
100 reactions | S3626 |
| SureFood® ALLERGEN Pine Nut |
The SureFood® ALLERGEN Pine Nut is a real-time PCR for the direct, qualitative detection of specific pine nut (Pinus spp.) DNA sequences in food. Each reaction contains an internal amplification control (IAC). Read more |
100 reactions | S3624 |
| SureFood® ALLERGEN Buckwheat |
The SureFood® ALLERGEN Buckwheat is a real-time PCR for the direct, qualitative and / or quantitative detection of specific buckwheat (Fagopyrum esculentum) DNA sequences. For the quantitative determination the use of the laboratory reference … Read more |
100 reactions | S3620 |
| SureFast® Mag PREP Food |
The kit is intended to be used for semi-automated isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® devices Maelstrom Switch 8 and Maelstrom 4810. Standardization and … Read more |
96 preparations | F1060 |
| SureFood® ALLERGEN 4plex Cereals |
The SureFood® ALLERGEN 4plex Cereals is a multiplex real-time PCR test for the direct, qualitative detection and differentiation of specific wheat (Triticum), barley (Hordeum vulgare) and rye (Secale cereale) DNA sequences in food. Each reaction … Read more |
100 reactions | S7006 |
| SureFood® Apricot |
The real-time PCR test detects DNA of apricot (Prunus armenica). Each reaction contains an internal amplification control (IAC). Read more |
100 reactions | S7007 |
| SureFood® ALLERGEN 4plex Macadamia / Brazil Nut / Pecan + IAC |
The SureFood® ALLERGEN 4plex Macadamia/Brazil Nut/Pecan + IAC is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific macadamia (Macadamia ternifolia), brazil nut (Bertholletia excelsa) and pecan (Carya … Read more |
100 reactions | S3403 |
| SureFood® ALLERGEN Oat |
The real-time PCR test detects DNA of oat (Avena sativa) qualitatively. Each reaction contains an internal amplification control (IAC). Read more |
100 reactions | S7004 |
| SureFood® ALLERGEN Mustard |
The SureFood® ALLERGEN Mustard is a real-time PCR for the direct, qualitative and / or quantitative detection of specific white mustard (Sinapis alba), indian mustard (Brassica juncea) und black mustard (Brassica nigra) DNA sequences according to … Read more |
100 reactions | S3609 |
| SureFood® ALLERGEN Lupin |
The real-time PCR test detects DNA of lupin (Lupinus spp.) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of the … Read more |
100 reactions | S3611 |
| SureFood® ALLERGEN Celery |
The real-time PCR test detects DNA of celery (Apium graveolens) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of … Read more |
100 reactions | S3605 |
| SureFood® ALLERGEN Molluscs |
The SureFood® ALLERGEN Molluscs is a real-time PCR for the direct, qualitative and / or quantitative detection of specific molluscs (mollusca) DNA sequences according to directive (EC) 1169/2011 in food. The kit was developed for the identification … Read more |
100 reactions | S3613 |
| SureFood® ALLERGEN Fish |
The SureFood® ALLERGEN Fish is a real-time PCR for the direct, qualitative and / or quantitative detection of specific fish DNA sequences according to directive (EC) 1169/2011. The group of fishes contains about 30000 species. Due to the large … Read more |
100 reactions | S3610 |
| SureFood® ALLERGEN Crustaceans |
The SureFood® ALLERGEN Crustaceans is a real-time PCR kit for the direct, qualitative and / or quantitative detection of specific crustaceans (Crustacea) DNA sequences according to directive (EC) 1169/2011. The crustacean family contains about … Read more |
100 reactions | S3612 |
| SureFood® ALLERGEN Sesame |
The real-time PCR test detects DNA of sesame (Sesamum indicum) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of … Read more |
100 reactions | S3608 |
| SureFood® ALLERGEN Walnut |
The SureFood® ALLERGEN Walnut is a real-time PCR for the direct, qualitative and / or quantitative detection of specific walnut (Juglans regia and Juglans nigra) DNA sequences according to directive (EC) 1169/2011. For the quantitative determination … Read more |
100 reactions | S3607 |
| SureFood® ALLERGEN Pistachio |
The SureFood® ALLERGEN Pistachio is a real-time PCR for the direct, qualitative and / or quantitative detection of specific pistachio (Pistacia vera) DNA sequences according to directive (EC) 1169/2011. For the quantitative determination the use of … Read more |
100 reactions | S3614 |
| SureFood® ALLERGEN Pecan |
The SureFood® ALLERGEN Pecan is a real-time PCR for the direct, qualitative and / or quantitative detection of specific pecan (Carya illinoinensis) DNA sequences according to directive (EC) 1169/2011. For the quantitative determination the use of … Read more |
100 reactions | S3618 |
| SureFood® ALLERGEN Peanut |
The real-time PCR test detects DNA of peanut (Arachis hypogaea) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of … Read more |
100 reactions | S3603 |
| SureFood® ALLERGEN Macadamia |
The real-time PCR test detects DNA of macadamia (Macadamia ternifolia) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the … Read more |
100 reactions | S3616 |
| SureFood® ALLERGEN Hazelnut |
The real-time PCR test detects DNA of hazelnut (Corylus avellana, Corylus maxima) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative … Read more |
100 reactions | S3602 |
| SureFood® ALLERGEN Cashew |
The real-time PCR test detects DNA of cashew (Anacardium occidentale) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the … Read more |
100 reactions | S3615 |
| SureFood® ALLERGEN Brazil Nut |
The SureFood® ALLERGEN Brazil Nut is a real-time PCR for the direct, qualitative and / or quantitative detection of specific brazil nut (Bertholletia excelsa) DNA sequences according to directive (EC) 1169/2011. For the quantitative determination … Read more |
100 reactions | S3617 |
| SureFood® ALLERGEN Almond |
The real-time PCR test detects DNA of almond (Prunus dulcis) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of … Read more |
100 reactions | S3604 |
| SureFood® ALLERGEN Soya |
The real-time PCR test detects soya DNA (Glycine max) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of the … Read more |
100 reactions | S3601 |
| SureFood® ALLERGEN Gluten |
The SureFood® ALLERGEN Gluten is a real-time PCR for the direct, qualitative and / or quantitative detection of specific gluten-containing cereals including wheat (Triticum spp.), rye (Secale cereale), barley (Hordeum vulgare) and oat (Avena sativa) … Read more |
100 reactions | S3606 |
| SureFood® ALLERGEN 4plex Soya / Celery / Mustard + IAC |
The SureFood® ALLERGEN 4plex Soya / Celery / Mustard + IAC is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific celery (Apium graveolens), mustard (Brassica carinata, Brassica juncea, Brassica nigra, … Read more |
100 reactions | S3401 |
| SureFood® QUANTARD Allergen 40 |
SureFood® QUANTARD Allergen 40 is a laboratory reference material developed for quantification in the range of 1 – 400 mg allergenic substance / kg food. It is verified in combination with the DNA extraction method SureFood® PREP Advanced … Read more |
1x 2 g | S3301 |
| SureFood® ALLERGEN 4plex Peanut / Hazelnut / Walnut + IAC |
The SureFood® ALLERGEN 4plex Peanut/Hazelnut/Walnut+IAC is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific Peanut (Arachis hypogaea), Hazelnut (Corylus maxima) and Walnut (Juglans regia, Juglans nigra) … Read more |
100 reactions | S3402 |
FAQ
A food allergy is an overreaction of the immune system to certain components of foods, usually proteins. The body mistakenly identifies these normally harmless substances as dangerous and produces specific antibodies (IgE antibodies).
Upon repeated contact with the allergy triggering food, these antibodies cause an allergic reaction.
Typical symptoms of a food allergy include:
- Itching
- Skin rash or hives
- Swelling in the mouth and throat area
- Breathing difficulties or shortness of breath
In severe cases, a life threatening allergic shock (anaphylaxis) can occur.
Common triggers of food allergies include:
- Nuts (e.g., almonds, hazelnuts)
- Peanuts
- Milk
- Eggs
- Soy
No, lactose intolerance is not an allergy but a food intolerance. Food intolerances are non allergic reactions to certain foods and are classified as metabolic disorders. They occur when an enzyme defect or enzyme deficiency prevents the body from properly processing ingested or released substances.
In the case of lactose intolerance, the body lacks partially or completely the enzyme lactase, which is needed to break down milk sugar (lactose). If lactose cannot be sufficiently digested, it passes undigested into the large intestine. There, the unprocessed lactose causes symptoms such as bloating, abdominal pain, or diarrhea.
Symptoms such as abdominal pain, diarrhea, or nausea can be triggered by certain foods. These symptoms may be caused by either a food intolerance or a food allergy. In both cases, it can help to adjust the diet and avoid specific foods.
In the case of a food allergy, the immune system overreacts. It perceives harmless substances, usually proteins, as dangerous and triggers a rapid and often strong reaction. Even very small amounts can be enough to cause symptoms.
A food intolerance, on the other hand, develops without the involvement of the immune system. Enzymes are often lacking, making it difficult for the body to process certain components of food. Symptoms occur with a delay and are generally not dangerous.
Yes, there are mandatory labelling requirements for allergens.
In the EU, the Food Information Regulation (LMIV) governs the labelling of 14 major allergens (Regulation (EU) No. 1169/2011). These must be clearly indicated on both pre packed and non pre packed foods.
Outside the EU, similar rules exist. The Codex Alimentarius (FAO/WHO) recommends worldwide labelling of the most important allergens and serves as a basis for legislation in many countries. In the United States, the Food Allergen Labelling and Consumer Protection Act (FALCPA) requires the labelling of currently 9 major allergens. Countries such as Canada, Australia, Japan, and New Zealand also have their own comparable regulations. However, there is no globally uniform standard.
The goal of all these regulations is to protect consumers.
Food products must be tested for allergens to protect the health of individuals with allergies. Even very small amounts of an allergen can trigger severe or life threatening reactions in sensitive individuals.
Allergen testing helps ensure that labelling information is accurate and that no unintended cross contamination occurs during production, storage, or transport. Manufacturers are also legally required to demonstrate compliance with labelling and threshold requirements (e.g., according to EU Regulation (EU) No. 1169/2011 and other international standards).
In addition, regular testing strengthens consumer trust, reduces product recalls, and supports companies in quality assurance. Allergen testing is therefore a central component of allergen management and essential for consumer protection and food safety.
In most countries, there are no defined threshold values for allergens in foods that apply to all products. Even small amounts can trigger severe reactions in sensitive individuals. Therefore, any allergen that is present must be clearly declared, as required by EU Regulation (EU) No. 1169/2011.
However, for dealing with cross contamination, some countries or organizations have developed reference values or guideline levels, such as the VITAL (Voluntary Incidental Trace Allergen Labelling) guidelines. These guidelines provide risk assessments for allergens based on clinical data about the minimum eliciting dose that can cause reactions in allergic individuals. This helps companies determine when a precautionary allergen label (PAL), such as “may contain traces of…”, is appropriate.
These guidelines are not legally binding, but they help minimize risk and support manufacturers in producing safe products for consumers. The goal is to offer maximum safety for people with allergies while avoiding unnecessary warning labels.
Since 2026, the Netherlands have a legally mandatory regulation regarding PAL. As part of this regulation, reference doses for each allergen were defined based on clinical data. In combination with the consumption size of a product, this allows the calculation of an action level for the respective food.
With the support of analytical testing and a risk assessment, manufacturers can determine whether the action level is exceeded and the product therefore requires PAL labelling, or whether consumption remains safe for the consumer without additional warnings.
For the analysis of allergens in food, different test systems are used, each with its own sensitivity and application range:
ELISA (enzyme immunoassay):
- most commonly used method in routine diagnostics
- detects specific allergenic proteins (the allergy triggering components)
- quantitative results
Rapid tests (lateral flow tests):
- primarily used on site for testing raw materials or production lines
- provide fast qualitative results
- for some allergens, quantitative rapid tests are also available
PCR methods (DNA based tests):
- detect DNA from allergen containing ingredients, even when the proteins have been altered by processing
- Multiplexing possible
Mass spectrometry (LC MS):
- highly specific
- can identify proteins and peptides even in heavily processed foods
- requires significant effort and cost
- demands extensive technical expertise
- involves complex sample preparation
Used only rarely within allergen management.
An ELISA is used when allergens in food need to be detected quantitatively. ELISA detects proteins and provides accurate concentration values. It is ideal for laboratory analyses, quality assurance, and traceability of raw materials or finished products. The overall testing process, including sample preparation and assay performance, takes about one hour. However, heavily processed foods can make protein detection more difficult.
For example, with RIDASCREEN®EASY Hazelnut and RIDASCREEN®EASY Mustard, hazelnut proteins or mustard proteins can be quantitatively measured in food products.
An LFD (lateral flow device / rapid test) is used when quick results are required. For example, it can be applied directly during incoming goods inspection of raw materials, for hygiene monitoring of production lines, for testing rinse water, or for on‑site product checks. LFDs are easy to use and provide qualitative results within minutes.
With the bioavid Lateral Flow Soy incl. Hook Line or the bioavid Lateral Flow Milk, soy or milk residues in food can be analyzed within 15 minutes.
A PCR test is used when allergen containing DNA needs to be detected. It is particularly useful for heavily processed foods, where proteins may be destroyed, or for allergens that are difficult to detect with immunological tests (ELISA, LFD) due to cross reactivities of the assay (e.g. celery or mustard).
PCR is highly specific and sensitive, making it especially suitable for controlled laboratory analyses and traceability.
Real-time PCR supports screening, quantification, and multiplex analyses, allowing multiple parameters to be detected in a single run. The method is also ideal for confirmation testing and advanced species identification.
The RIDASCREEN® Allergen kits from R Biopharm enable reliable and sensitive detection of numerous food allergens.
Detectable allergens:
The RIDA®QUICK and bioavid Allergen Kits from R-Biopharm enable reliable and sensitive detection of numerous food allergens.
Detectable allergens:
- Gluten (qualitative and quantitative)
- Soy (with hook line and without hook line)
- Milk
- Casein
- Coconut
- Almond
- Brazil nut
- Mustard
- Hazelnut
- Macadamia
- Peanut
- Walnut
- Egg
- Sesame
- Cashew
- Pistachio
- Crustacean
The SureFood® Allergen PCR kits from R‑Biopharm enable reliable and sensitive detection of a wide range of food allergens. These kits support qualitative and quantitative analysis of the following allergens:
Detectable allergens:
- Almond
- Brazil nut
- Buckwheat
- Cashew
- Celery
- Crustaceans
- Fish
- Gluten‑containing cereals: wheat, barley, rye, oat
- Hazelnut
- Lupin
- Macadamia
- Molluscs
- Mustard
- Peanut
- Pistachio
- Sesame
- Soya
- Walnut
- Coconut
- Beechnut
- Pine nut
- Shea nut
For quantitative analysis, the reference material SureFood® QUANTARD Allergen 40 is recommended.
For LOD detection, SureFood® QUANTARD Allergen 1 ppm is available.
For broad allergen screening, the multiplex kits are especially suitable, as they allow simultaneous detection of multiple allergens.
Food products consist of many different components such as fats, carbohydrates, and proteins, as well as vitamins and trace elements. Since each food has a different composition, this so called matrix can influence the analysis of allergens. Such matrix effects may be unspecific (e.g., interferences) or specific (e.g., cross reactions with the test system).
In addition, processing steps can alter the structure of proteins that are detected in immunological tests:
- High temperatures during baking or roasting can denature proteins, meaning they lose their natural structure.
- Fermentation, as in beer production, can break down proteins.
Both processes affect how well allergenic proteins can be detected.
In principle, any food can be tested for allergens, but it must first be evaluated to ensure it is suitable for the specific test system before being used in routine analysis. It is particularly important that the sample is homogeneous, meaning well mixed, to achieve reliable results.
In principle, allergens can also be detected in hydrolyzed and fermented foods. However, it must be taken into account that proteins may be partially or completely broken-down during hydrolysis or fermentation. Immunological tests can only recognize these altered structures under certain conditions.
In sandwich ELISAs (antibody-antigen-antibody), only larger protein fragments containing at least two epitopes can be detected. If proteins are broken-down into many small fragments, they can only be detected using competitive test systems, where a single epitope is sufficient for recognition. For example, RIDASCREEN® β‑Lactoglobulin is a competitive ELISA suitable for the quantitative determination of β‑lactoglobulin in hydrolyzed milk products.
Because the actual degree of hydrolysis or fermentation is often unknown for many products, choosing the appropriate test method can be challenging. Therefore, samples with an unknown level of hydrolysis should always be analyzed using both test systems (sandwich and competitive). The higher result is considered the more reliable one.
So-called incurred samples are food samples in which an allergen is intentionally incorporated into the product before processing. Unlike spiked samples, where the allergen is added only to the finished end product, the allergenic proteins in incurred samples pass through the entire manufacturing process of a food.
This approach realistically simulates natural contamination during production. Because proteins undergo structural changes during processing steps such as mixing, heating, baking, roasting, or fermentation, these changes affect how well they can be detected by a test method. For this reason, incurred samples are considered realistic and representative test materials.
A typical example is a cookie in which the dough is homogenously mixed with 10 mg of hazelnut flour before baking. During baking, the high temperatures modify the structure of the hazelnut proteins, mirroring the changes that occur in real industrial processes.
Official bodies such as the AOAC also recognize the importance of incurred samples and updated their guidelines for validating immunological test systems for food allergens accordingly in 2023.
The test systems RIDASCREEN®EASY Hazelnut, RIDASCREEN®EASY Mustard, and RIDASCREEN®EASY Crustacean belong to the RIDASCREEN®EASY Allergen ELISA product line, which has been validated using incurred samples in line with the latest AOAC requirements.
When analyzing allergens, various factors must be taken into account to correctly interpret the results. A central aspect is the test system and the result format: the analysis may have been carried out using an immunological method such as ELISA or LFD, or with PCR. Depending on the method, the result may be qualitative (result output yes/no) or presented as a quantitative measurement value, which greatly influences its significance.
It is equally important to consider what is actually being detected. Immunological tests identify proteins or protein fragments, whereas PCR detects only DNA. This means that a positive PCR result does not automatically indicate that the allergen is present in its protein active form in the food.
The type of sample also plays a significant role. Raw materials, finished products, hygiene monitoring samples, spiked samples, or processed foods differ considerably in their composition and therefore in their analyzability. In addition, matrix effects, such as interferences or cross reactions, can influence measurement results.
To obtain reliable results, the sample itself must also be carefully examined. Good homogeneity and suitable sample extraction are essential, as inhomogeneities or inadequate extraction conditions can lead to distorted results.
Finally, the measurement range of the respective test system must be considered. This includes the limit of detection (LoD) and the limit of quantification (LoQ). Values below these limits should not be overinterpreted, as they cannot be reliably quantified by the method.
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