EuroProxima Colistin

Art. No.: 5151COL

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EuroProxima Colistin

Art. No.: 5151COL

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Intended use

A competitive enzyme immunoassay for screening and quantitative analysis of colistin in various matrices.

General Information

Colistin A and B are polypeptide antibiotics that have been used in medicine and veterinary for over 50 years. The European Medicines Agency has proposed to reduce the use of the antibiotic drug colistin in food producing animals. For several food matrices maximum residue limits (MRLs) for colistin are set in the European Union: 150 μg/kg (ppb) for muscle, fat and liver; 200 μg/kg for kidney, 50 μg/l for milk; 300 μg/kg for eggs.

The Colistin ELISA is a competitive enzyme immunoassay based on specific antibodies directed against colistin.

Accessories

Specifications
Art. No5151COL
Test formatMicrotiter plate with 96 wells (12 strips with 8 wells each).
Sample preparationFor the mentioned matrices, fast and efficient methods are included in the test kit description.
Incubation time1 h, RT
LOD (Detection Limit)Milk 4.3 µg/l, egg 22 µg/kg, chicken 11.7 µg/kg, pork 7.6 µg/kg, beef 14.6 µg/kg, liver 21.3 µg/kg, fish 12.5 µg/kg, feed 24.1 µg/kg
Detection CapabilityMilk 10 µg/l, egg 30 µg/kg, chicken 30 µg/kg, pork 30 µg/kg, beef 30 µg/kg, liver 30 µg/kg, fish 30 µg/kg, feed 30 µg/kg
Validated matrices

Milk, egg, chicken, pork, beef, liver, fish and feed.

Detected analyte

Colistin in milk, egg, chicken, pork, beef, liver, fish and feed.

EvaluationMicrotiter plate spectrophotometer (450 nm)
Files
InstructionsEnglish
MSDS5151col-1.zip (English)