ELISA

Efficient immunoassays for food analysis

Technologies

ELISA

El ensayo por inmunoabsorción ligado a enzimas (ELISA) es un método de prueba basado en anticuerpos. Esta tecnología de uso extendido es sensible, rápida y fiable.

Con los kits de ensayos ELISA, R-Biopharm ofrece un método analítico bien establecido que satisface los requisitos de los laboratorios modernos. Los ensayos RIDASCREEN® demuestran una sensibilidad elevada y se caracterizan por su alta solidez. Para el cribado urgente de diversos productos, se ofrecen los ensayos RIDASCREEN®FAST. Estos ensayos permiten analizar muestras de alimentos y piensos en un plazo máximo de 30 minutos, incluida la preparación de muestras.

Principios del ensayo ELISA

Los anticuerpos son proteínas producidas en las células plasmáticas de los vertebrados como parte del sistema inmunitario adaptativo frente a estructuras (antígenos) que el cuerpo reconoce como elementos extraños. Los anticuerpos se enlazan a los antígenos correspondientes utilizando un patrón diferenciado de interacciones iónicas e hidrofóbicas, enlaces de puente de hidrógeno y fuerzas de Van-der-Waals. La interacción entre el anticuerpo y el antígeno correspondiente es selectiva y muy específica, similar a la de una cerradura y una llave.

El ensayo por inmunoabsorción ligado a enzimas se basa en este reconocimiento anticuerpo-antígeno selectivo y específico Se han establecido muchos formatos de ensayos ELISA cualitativos y cuantitativos. La realización de un ensayo ELISA requiere como mínimo un anticuerpo específico para un antígeno concreto. De acuerdo con el método básico, uno de los componentes inmunológicos se inmoviliza en una fase sólida, en las cavidades de la placa de microtitulación. El analito de la muestra interactúa con el sistema anticuerpo-antígeno. Esta interacción se puede visualizar mediante enzimas, enlazadas a antígenos o anticuerpos secundarios, e indica si se ha producido un enlace antígeno-anticuerpo. La enzima enlazada convierte un sustrato agregado, lo que da lugar a un cambio de color, que se puede medir mediante un espectrofotómetro.

ELISA tipo sandwich y ELISA competitivo

Mientras que el ensayo ELISA tipo sandwich detecta solo las proteínas intactas y fragmentos de gran tamaño con un mínimo de dos puntos de enlace, un ensayo ELISA competitivo es capaz de detectar fragmentos de proteína grandes y pequeños con solo un punto de enlace. Por este motivo, el ensayo ELISA competitivo también se puede utilizar en productos alimentarios muy procesados.

Good ELISA practice (Prácticas recomendadas para ELISA)

El manual Prácticas recomendadas para ELISA proporciona un amplio resumen para analistas tanto principiantes como avanzados con el fin de mejorar la calidad del análisis ELISA realizado. Proporciona las directrices que le ayudarán a establecer unas condiciones razonables del entorno de trabajo, así como las condiciones de uso que se deberán cumplir para conseguir el mejor rendimiento posible al utilizar los kits de ensayo ELISA de R-Biopharm.

Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
EuroProxima Plus Bovine Rennet Whey

EuroProxima Plus Bovine Rennet Whey is a competitive enzyme immunoassay for screening of the presence of bovine rennet whey in bovine milk and in milk of other species and sources.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5171BRW
EuroProxima Plus Cheese Fraud

EuroProxima Plus Cheese Fraud is a competitive enzyme immunoassay for screening of the presence of bovine milk in cheese of other species and sources.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5171BKCC
EuroProxima Plus Lactoferrin

EuroProxima Plus Lactoferrin is a competitive enzyme immunoassay for screening and quantitative analysis of lactoferrin in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5091LFER
ELISA-TEK™ Cooked Meat Species Kit

Assay for the positive identification of species content (various) in cooked samples: ELISA-TEK™ Cooked Mixed Species Kit: customized (Art. No. 510601) ELISA-TEK™ Cooked Meat 3 Species Kit: beef, pork, poultry (Art. No. 510603) ELISA-TEK™ …

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96 determinations 5106**
ELISA-TEK™ Raw Meat Species Kit

Assay for the positive identification of species content (various) in raw samples: ELISA-TEK™ Raw Mixed Species Kit: customized (Art. No. 510501) ELISA-TEK™ Raw 3 Species Kit: cow, pig, poultry (Art. No. 510503) ELISA-TEK™ Raw 4 Species Kit: …

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96 determinations 5105**
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ProductDescriptionNo. of tests/amountArt. No.
RIDASCREEN® Egg

RIDASCREEN® Egg is a sandwich enzyme immunoassay developed for the quantitative analysis of native and processed hen´s egg in foods. The following foods were examined as representatives of the group of products including baked goods, sweets, …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R6411
RIDASCREEN®FAST Gliadin sensitive

RIDASCREEN®FAST Gliadin sensitive is a sandwich enzyme immunoassay for the determination of prolamins from gluten containing cereals in as gluten-free declared food (cookies, rice flour, oat flour, sausages, quinoa flour, chocolate, millet flour).

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R7051
RIDASCREEN® Gliadin

RIDASCREEN® Gliadin (Art. No. R7001) is a R5 sandwich enzyme immunoassay based on specific monoclonal antibody to celiac toxic amino acid prolamin sequences to determine gliadin as a measure of gluten in food. It is applicable for the quantitative …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R7001
RIDASCREEN®FAST Cashew

The RIDASCREEN®FAST Cashew is a sandwich enzyme immunoassay developed for the quantitative analysis of Cashew in food. Exemplarily for the food groups pastries and sweets have been tested during the assay’s development: cookies, chocolate and ice …

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R6872
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ProductDescriptionNo. of tests/amountArt. No.
RIDASCREEN® T-2 / HT-2 Toxin

RIDASCREEN® T-2 / HT-2 Toxin is a competitive enzyme immunoassay for the quantitative analysis of the sum of T-2 and HT-2 toxin in oats, corn (maize), barley and wheat.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R3805
RIDASCREEN® Zearalenon

RIDASCREEN® Zearalenon is a competitive enzyme immunoassay for the quantitative analysis of zearalenone residues in cereals, feed, beer, serum and urine.

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Microtiter plate with 96 wells (12 strips with 8 wells each). R1401
RIDASCREEN® DON

RIDASCREEN® DON is a competitive enzyme immunoassay for the quantitative analysis of DON in cereals, malt, feed, beer and wort.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R5906
RIDASCREEN® T-2 Toxin

RIDASCREEN® T-2 Toxin is a competitive enzyme immunoassay for the quantitative determination of T-2 toxin in cereals and feed.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R3801
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ProductDescriptionNo. of tests/amountArt. No.
EuroProxima Trenbolone

This competitive enzyme immunoassay is used for the quantitative determination of trenbolone in urine, liver and tissue.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5081TRENBO
EuroProxima Florfenicol-amine

A competitive enzyme immunoassay for screening and quantitative analysis of florfenicol-amine in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5091FLOA
EuroProxima Neomycin

A competitive enzyme immunoassay for screening and quantitative analysis of neomycin in milk, milk powder, tissue, honey, serum/plasma and urine samples.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5111NEO
EuroProxima Oxytetracycline

A competitive enzyme immunoassay for screening and quantitative analysis of oxytetracyclines in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5091OTC
EuroProxima Tylosin

EuroProxima Tylosin is a competitive enzyme immunoassay for screening and quantitative analysis of tylosin in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5151TYL
EuroProxima Virginiamycin

EuroProxima Virginiamycin is a competitive enzyme immunoassay for quantitative analysis of virginiamycin in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5151VIG
EuroProxima Fluoroquinolones

EuroProxima Fluoroquinolones is a competitive enzyme immunoassay for screening and quantitative analysis of a broad range of fluoroquinolones in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5101FLUQG
EuroProxima Fluoroquinolones II

EuroProxima Fluoroquinolones II is a competitive enzyme immunoassay for screening and quantitative analysis of fluoroquinolones in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5101FLUQII
EuroProxima Promazine, generic

EuroProxima Promazine, generic is a competitive enzyme immunoassay for screening and quantitative analysis of several promazines in tissue, liver, urine and kidney.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5201PROM
EuroProxima Malachite Green Total/Crystal Violet

EuroProxima Malachite Green Total/Crystal Violet is a competitive enzyme immunoassay for quantitative analysis of malachite green, leucomalachite green, crystal violet and leucocrystal violet in fish and shrimp.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5161MGT
EuroProxima Azaperone-Azaperol

EuroProxima Azaperone-Azaperol is a competitive enzyme immunoassay for screening and quantitative analysis of azaperone/azaperol in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5201AZA
EuroProxima Carazolol

EuroProxima Carazolol is a competitive enzyme immunoassay for screening and quantitative analysis of carazolol in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5201CARA
EuroProxima Corticosteroid

This competitive enzyme immunoassay is used for the quantitative determination of corticosteroids in milk, urine, muscle, liver and feed.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5081COR
EuroProxima Progesterone

This competitive enzyme immunoassay is used for the quantitative determination of progesterone in milk and serum.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5081PROG
EuroProxima Stanozolol

This competitive enzyme immunoassay is used for the quantitative determination of stanozolol in urine and faeces.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5081STAN
EuroProxima Triamcinolone

This competitive enzyme immunoassay is used for the quantitative determination of triamcinolone in urine.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5081TRIA
EuroProxima Saxitoxin

EuroProxima Saxitoxin is a competitive enzyme immunoassay for the screening and quantitative analysis of the shellfish poison saxitoxin in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5191SAXI
EuroProxima Lincomycin

A competitive enzyme immunoassay for screening and quantitative analysis of lincomycin in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5151LIN
EuroProxima Gentamicin

A competitive enzyme immunoassay for screening and quantitative analysis of gentamicin in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5111GEN
EuroProxima Flumequine

EuroProxima Flumequine is a competitive enzyme immunoassay for screening and quantitative analysis of flumequine in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5101FLUM
EuroProxima Erythromycin

EuroProxima Erythromycin is a competitive enzyme immunoassay for screening and quantitative analysis of erythromycin in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5151ERY
EuroProxima Domoic Acid

EuroProxima Domoic Acid is a competitive enzyme immunoassay for quantitative analysis of domoic acid in scallop, mussel and oyster samples.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5191DOMO
EuroProxima Dimetridazole

EuroProxima Dimetridazole is a competitive enzyme immunoassay for quantitative analysis of dimetridazole in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5091DIME
EuroProxima Colistin

A competitive enzyme immunoassay for screening and quantitative analysis of colistin in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5151COL
EuroProxima Ivermectin

EuroProxima Ivermectin is an unique competitive enzyme immunoassay (ELISA) for the detection of ivermectin in urine, tissue and milk samples.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5141IVER
EuroProxima Diethylstilbestrol (DES)

A competitive enzyme immunoassay for screening and quantitative analysis of diethylstilbestrol (DES) in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5081DES
EuroProxima Penicillin

A competitive enzyme immunoassay for screening and quantitative analysis of penicillins in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5091PEN
RIDASCREEN® Tetracyclin

RIDASCREEN® Tetracyclin is a competitive enzyme immunoassay for the quantitative analysis of tetracyclins in milk, milk powder, cheese, butter, dairy products (curd, yoghurt (plain/with fruits), kefir, cream, sour cream) honey, meat, sausage, fish, …

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Microtiter plate with 96 wells (12 strips with 8 wells each) R3505
RIDASCREEN® Chloramphenicol

RIDASCREEN® Chloramphenicol is a competitive enzyme immunoassay for the quantitative analysis of chloramphenicol in milk, milk powder and dairy products, honey and royal jelly, meat, fish, shrimp, eggs, urine (also chloramphenicol glucuronide), …

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Microtiter plate with 96 wells (12 strips with 8 wells each) R1511
RIDASCREEN® Sulfamethazin

RIDASCREEN® Sulfamethazin is a competitive enzyme immunoassay for the quantitative determination of sulfamethazine in milk, meat, honey, liver, kidney, fish, shrimp and egg.

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Microtiter plate with 96 wells (12 strips with 8 wells each). R3011
RIDASCREEN® Streptomycin

RIDASCREEN® Streptomycin is a competitive enzyme immunoassay for the quantitative analysis of streptomycin in milk and milk powder, honey, meat, liver, kidney, shrimp and apple juice.

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Microtiter plate with 96 wells (12 strips with 8 wells each). R3104
RIDASCREEN® Bacitracin

RIDASCREEN® Bacitracin is a competitive enzyme immunoassay for the quantitative analysis of bacitracin in milk, meat, eggs, feed and urine.

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Microtiter plate with 96 wells (12 strips with 8 removable wells each). R2901
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