ELISA
Efficient immunoassays for food analysis
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ELISA
El ensayo por inmunoabsorción ligado a enzimas (ELISA) es un método de prueba basado en anticuerpos. Esta tecnología de uso extendido es sensible, rápida y fiable.
Con los kits de ensayos ELISA, R-Biopharm ofrece un método analítico bien establecido que satisface los requisitos de los laboratorios modernos. Los ensayos RIDASCREEN® demuestran una sensibilidad elevada y se caracterizan por su alta solidez. Para el cribado urgente de diversos productos, se ofrecen los ensayos RIDASCREEN®FAST. Estos ensayos permiten analizar muestras de alimentos y piensos en un plazo máximo de 30 minutos, incluida la preparación de muestras.
Principios del ensayo ELISA
Los anticuerpos son proteínas producidas en las células plasmáticas de los vertebrados como parte del sistema inmunitario adaptativo frente a estructuras (antígenos) que el cuerpo reconoce como elementos extraños. Los anticuerpos se enlazan a los antígenos correspondientes utilizando un patrón diferenciado de interacciones iónicas e hidrofóbicas, enlaces de puente de hidrógeno y fuerzas de Van-der-Waals. La interacción entre el anticuerpo y el antígeno correspondiente es selectiva y muy específica, similar a la de una cerradura y una llave.
El ensayo por inmunoabsorción ligado a enzimas se basa en este reconocimiento anticuerpo-antígeno selectivo y específico Se han establecido muchos formatos de ensayos ELISA cualitativos y cuantitativos. La realización de un ensayo ELISA requiere como mínimo un anticuerpo específico para un antígeno concreto. De acuerdo con el método básico, uno de los componentes inmunológicos se inmoviliza en una fase sólida, en las cavidades de la placa de microtitulación. El analito de la muestra interactúa con el sistema anticuerpo-antígeno. Esta interacción se puede visualizar mediante enzimas, enlazadas a antígenos o anticuerpos secundarios, e indica si se ha producido un enlace antígeno-anticuerpo. La enzima enlazada convierte un sustrato agregado, lo que da lugar a un cambio de color, que se puede medir mediante un espectrofotómetro.
ELISA tipo sandwich y ELISA competitivo
Mientras que el ensayo ELISA tipo sandwich detecta solo las proteínas intactas y fragmentos de gran tamaño con un mínimo de dos puntos de enlace, un ensayo ELISA competitivo es capaz de detectar fragmentos de proteína grandes y pequeños con solo un punto de enlace. Por este motivo, el ensayo ELISA competitivo también se puede utilizar en productos alimentarios muy procesados.
Good ELISA practice (Prácticas recomendadas para ELISA)
El manual Prácticas recomendadas para ELISA proporciona un amplio resumen para analistas tanto principiantes como avanzados con el fin de mejorar la calidad del análisis ELISA realizado. Proporciona las directrices que le ayudarán a establecer unas condiciones razonables del entorno de trabajo, así como las condiciones de uso que se deberán cumplir para conseguir el mejor rendimiento posible al utilizar los kits de ensayo ELISA de R-Biopharm.
Product portfolio
Product | Description | No. of tests/amount | Art. No. |
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EuroProxima Plus Bovine Rennet Whey |
EuroProxima Plus Bovine Rennet Whey is a competitive enzyme immunoassay for screening of the presence of bovine rennet whey in bovine milk and in milk of other species and sources. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5171BRW |
EuroProxima Plus Cheese Fraud |
EuroProxima Plus Cheese Fraud is a competitive enzyme immunoassay for screening of the presence of bovine milk in cheese of other species and sources. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5171BKCC |
EuroProxima Plus Lactoferrin |
EuroProxima Plus Lactoferrin is a competitive enzyme immunoassay for screening and quantitative analysis of lactoferrin in various matrices. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5091LFER |
ELISA-TEK™ Cooked Meat Species Kit |
Assay for the positive identification of species content (various) in cooked samples: ELISA-TEK™ Cooked Mixed Species Kit: customized (Art. No. 510601) ELISA-TEK™ Cooked Meat 3 Species Kit: beef, pork, poultry (Art. No. 510603) ELISA-TEK™ … Lee más |
96 determinations | 5106** |
ELISA-TEK™ Raw Meat Species Kit |
Assay for the positive identification of species content (various) in raw samples: ELISA-TEK™ Raw Mixed Species Kit: customized (Art. No. 510501) ELISA-TEK™ Raw 3 Species Kit: cow, pig, poultry (Art. No. 510503) ELISA-TEK™ Raw 4 Species Kit: … Lee más |
96 determinations | 5105** |
Product | Description | No. of tests/amount | Art. No. |
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RIDASCREEN® Egg |
RIDASCREEN® Egg is a sandwich enzyme immunoassay developed for the quantitative analysis of native and processed hen´s egg in foods. The following foods were examined as representatives of the group of products including baked goods, sweets, … Lee más |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R6411 |
RIDASCREEN®FAST Gliadin sensitive |
RIDASCREEN®FAST Gliadin sensitive is a sandwich enzyme immunoassay for the determination of prolamins from gluten containing cereals in as gluten-free declared food (cookies, rice flour, oat flour, sausages, quinoa flour, chocolate, millet flour). Lee más |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R7051 |
RIDASCREEN® Gliadin |
RIDASCREEN® Gliadin (Art. No. R7001) is a R5 sandwich enzyme immunoassay based on specific monoclonal antibody to celiac toxic amino acid prolamin sequences to determine gliadin as a measure of gluten in food. It is applicable for the quantitative … Lee más |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R7001 |
RIDASCREEN®FAST Cashew |
The RIDASCREEN®FAST Cashew is a sandwich enzyme immunoassay developed for the quantitative analysis of Cashew in food. Exemplarily for the food groups pastries and sweets have been tested during the assay’s development: cookies, chocolate and ice … Lee más |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R6872 |
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
RIDASCREEN® T-2 / HT-2 Toxin |
RIDASCREEN® T-2 / HT-2 Toxin is a competitive enzyme immunoassay for the quantitative analysis of the sum of T-2 and HT-2 toxin in oats, corn (maize), barley and wheat. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R3805 |
RIDASCREEN® Zearalenon |
RIDASCREEN® Zearalenon is a competitive enzyme immunoassay for the quantitative analysis of zearalenone residues in cereals, feed, beer, serum and urine. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each). | R1401 |
RIDASCREEN® DON |
RIDASCREEN® DON is a competitive enzyme immunoassay for the quantitative analysis of DON in cereals, malt, feed, beer and wort. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R5906 |
RIDASCREEN® T-2 Toxin |
RIDASCREEN® T-2 Toxin is a competitive enzyme immunoassay for the quantitative determination of T-2 toxin in cereals and feed. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R3801 |
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
EuroProxima Trenbolone |
This competitive enzyme immunoassay is used for the quantitative determination of trenbolone in urine, liver and tissue. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081TRENBO |
EuroProxima Florfenicol-amine |
A competitive enzyme immunoassay for screening and quantitative analysis of florfenicol-amine in various matrices. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5091FLOA |
EuroProxima Neomycin |
A competitive enzyme immunoassay for screening and quantitative analysis of neomycin in milk, milk powder, tissue, honey, serum/plasma and urine samples. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5111NEO |
EuroProxima Oxytetracycline |
A competitive enzyme immunoassay for screening and quantitative analysis of oxytetracyclines in various matrices. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5091OTC |
EuroProxima Tylosin |
EuroProxima Tylosin is a competitive enzyme immunoassay for screening and quantitative analysis of tylosin in various matrices. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5151TYL |
EuroProxima Virginiamycin |
EuroProxima Virginiamycin is a competitive enzyme immunoassay for quantitative analysis of virginiamycin in various matrices. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5151VIG |
EuroProxima Fluoroquinolones |
EuroProxima Fluoroquinolones is a competitive enzyme immunoassay for screening and quantitative analysis of a broad range of fluoroquinolones in various matrices. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5101FLUQG |
EuroProxima Fluoroquinolones II |
EuroProxima Fluoroquinolones II is a competitive enzyme immunoassay for screening and quantitative analysis of fluoroquinolones in various matrices. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5101FLUQII |
EuroProxima Promazine, generic |
EuroProxima Promazine, generic is a competitive enzyme immunoassay for screening and quantitative analysis of several promazines in tissue, liver, urine and kidney. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5201PROM |
EuroProxima Malachite Green Total/Crystal Violet |
EuroProxima Malachite Green Total/Crystal Violet is a competitive enzyme immunoassay for quantitative analysis of malachite green, leucomalachite green, crystal violet and leucocrystal violet in fish and shrimp. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5161MGT |
EuroProxima Azaperone-Azaperol |
EuroProxima Azaperone-Azaperol is a competitive enzyme immunoassay for screening and quantitative analysis of azaperone/azaperol in various matrices. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5201AZA |
EuroProxima Carazolol |
EuroProxima Carazolol is a competitive enzyme immunoassay for screening and quantitative analysis of carazolol in various matrices. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5201CARA |
EuroProxima Corticosteroid |
This competitive enzyme immunoassay is used for the quantitative determination of corticosteroids in milk, urine, muscle, liver and feed. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081COR |
EuroProxima Progesterone |
This competitive enzyme immunoassay is used for the quantitative determination of progesterone in milk and serum. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081PROG |
EuroProxima Stanozolol |
This competitive enzyme immunoassay is used for the quantitative determination of stanozolol in urine and faeces. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081STAN |
EuroProxima Triamcinolone |
This competitive enzyme immunoassay is used for the quantitative determination of triamcinolone in urine. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081TRIA |
EuroProxima Saxitoxin |
EuroProxima Saxitoxin is a competitive enzyme immunoassay for the screening and quantitative analysis of the shellfish poison saxitoxin in various matrices. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5191SAXI |
EuroProxima Lincomycin |
A competitive enzyme immunoassay for screening and quantitative analysis of lincomycin in various matrices. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5151LIN |
EuroProxima Gentamicin |
A competitive enzyme immunoassay for screening and quantitative analysis of gentamicin in various matrices. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5111GEN |
EuroProxima Flumequine |
EuroProxima Flumequine is a competitive enzyme immunoassay for screening and quantitative analysis of flumequine in various matrices. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5101FLUM |
EuroProxima Erythromycin |
EuroProxima Erythromycin is a competitive enzyme immunoassay for screening and quantitative analysis of erythromycin in various matrices. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5151ERY |
EuroProxima Domoic Acid |
EuroProxima Domoic Acid is a competitive enzyme immunoassay for quantitative analysis of domoic acid in scallop, mussel and oyster samples. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5191DOMO |
EuroProxima Dimetridazole |
EuroProxima Dimetridazole is a competitive enzyme immunoassay for quantitative analysis of dimetridazole in various matrices. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5091DIME |
EuroProxima Colistin |
A competitive enzyme immunoassay for screening and quantitative analysis of colistin in various matrices. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5151COL |
EuroProxima Ivermectin |
EuroProxima Ivermectin is an unique competitive enzyme immunoassay (ELISA) for the detection of ivermectin in urine, tissue and milk samples. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5141IVER |
EuroProxima Diethylstilbestrol (DES) |
A competitive enzyme immunoassay for screening and quantitative analysis of diethylstilbestrol (DES) in various matrices. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081DES |
EuroProxima Penicillin |
A competitive enzyme immunoassay for screening and quantitative analysis of penicillins in various matrices. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5091PEN |
RIDASCREEN® Tetracyclin |
RIDASCREEN® Tetracyclin is a competitive enzyme immunoassay for the quantitative analysis of tetracyclins in milk, milk powder, cheese, butter, dairy products (curd, yoghurt (plain/with fruits), kefir, cream, sour cream) honey, meat, sausage, fish, … Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R3505 |
RIDASCREEN® Chloramphenicol |
RIDASCREEN® Chloramphenicol is a competitive enzyme immunoassay for the quantitative analysis of chloramphenicol in milk, milk powder and dairy products, honey and royal jelly, meat, fish, shrimp, eggs, urine (also chloramphenicol glucuronide), … Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R1511 |
RIDASCREEN® Sulfamethazin |
RIDASCREEN® Sulfamethazin is a competitive enzyme immunoassay for the quantitative determination of sulfamethazine in milk, meat, honey, liver, kidney, fish, shrimp and egg. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each). | R3011 |
RIDASCREEN® Streptomycin |
RIDASCREEN® Streptomycin is a competitive enzyme immunoassay for the quantitative analysis of streptomycin in milk and milk powder, honey, meat, liver, kidney, shrimp and apple juice. Lee más |
Microtiter plate with 96 wells (12 strips with 8 wells each). | R3104 |
RIDASCREEN® Bacitracin |
RIDASCREEN® Bacitracin is a competitive enzyme immunoassay for the quantitative analysis of bacitracin in milk, meat, eggs, feed and urine. Lee más |
Microtiter plate with 96 wells (12 strips with 8 removable wells each). | R2901 |