ELISA
Des tests immunologiques efficaces pour l’analyse des denrées alimentaires
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ELISA
La technique ELISA (test d’immuno-absorption enzymatique) est une méthode à base d’anticorps. Très répandue, c’est une technologie sensible, rapide et fiable.
Avec ses kits de test ELISA, R-Biopharm s’appuie sur une méthode analytique bien établie qui répond aux exigences des laboratoires modernes. Les tests RIDASCREEN® font preuve d’une sensibilité élevée et se caractérisent par leur robustesse. Les tests RIDASCREEN®FAST permettent le criblage rapide de différents produits. Ils permettent d’analyser des échantillons de denrées alimentaires et d’aliments pour animaux en seulement 30 minutes, préparation des échantillons incluse.
Principe de la technique ELISA
Les anticorps sont des protéines produites par les cellules plasmatiques des vertébrés au sein du système immunitaire adaptatif en réponse à la présence de structures (antigènes) reconnues comme étrangères à l’organisme. Les anticorps se lient à leurs antigènes par différents moyens : interactions ioniques et hydrophobes, liaisons hydrogènes et forces de van der Waals. L’interaction entre l’anticorps et son antigène est sélective et hautement spécifique, comparable à une serrure et une clé.
Le dosage par immuno-absorption enzymatique est basé sur cette reconnaissance anticorps-antigène sélective et spécifique. Il existe de nombreux formats de tests ELISA qualitatifs ou quantitatifs. La réalisation d’un test ELISA implique au moins un anticorps spécifique pour un antigène donné. Le principe de base du test est d’immobiliser l’un de ces composants immunologiques sur une phase solide : les cavités d’une microplaque. L’analyte de l’échantillon interagit avec le système anticorps-antigène. Cette interaction peut être visualisée par les enzymes, liées à des anticorps ou antigènes secondaires, ce qui indique l’existence d’une liaison anticorps-antigène. Le substrat ajouté est converti par l’enzyme couplée, ce qui entraîne un changement de couleur mesurable à l’aide d’un spectrophotomètre.
Sandwich ELISA et ELISA compétitif
Alors que le sandwich ELISA ne détecte que les protéines intactes et les fragments de grande taille comprenant au moins deux sites de liaison, le test ELISA compétitif est capable de détecter des fragments protéiques de grande et petite taille avec un seul site de liaison. Le test ELISA compétitif peut donc être utilisé pour analyser des produits alimentaires hautement transformés.
Bonnes pratiques applicables aux tests ELISA
Les bonnes pratiques applicables aux tests ELISA sont destinées tant aux débutants qu’aux analystes expérimentés pour améliorer la qualité des analyses ELISA réalisées. Elles incluent des recommandations qui vous aideront à mettre en place une structure et des conditions d’utilisation raisonnables, lesquelles devront être respectées afin d’obtenir les meilleures performances possibles lors de l’utilisation des kits de test ELISA R-Biopharm.
Product portfolio
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
RIDASCREEN® Egg |
RIDASCREEN® Egg is a sandwich enzyme immunoassay developed for the quantitative analysis of native and processed hen´s egg in foods. The following foods were examined as representatives of the group of products including baked goods, sweets, … En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R6411 |
RIDASCREEN®FAST Gliadin sensitive |
RIDASCREEN®FAST Gliadin sensitive is a sandwich enzyme immunoassay for the determination of prolamins from gluten containing cereals in as gluten-free declared food (cookies, rice flour, oat flour, sausages, quinoa flour, chocolate, millet flour). En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R7051 |
RIDASCREEN® Gliadin |
RIDASCREEN® Gliadin (Art. No. R7001) is a R5 sandwich enzyme immunoassay based on specific monoclonal antibody to celiac toxic amino acid prolamin sequences to determine gliadin as a measure of gluten in food. It is applicable for the quantitative … En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R7001 |
RIDASCREEN®FAST Cashew |
The RIDASCREEN®FAST Cashew is a sandwich enzyme immunoassay developed for the quantitative analysis of Cashew in food. Exemplarily for the food groups pastries and sweets have been tested during the assay’s development: cookies, chocolate and ice … En savoir plus |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R6872 |
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
EuroProxima Plus Bovine Rennet Whey |
EuroProxima Plus Bovine Rennet Whey is a competitive enzyme immunoassay for screening of the presence of bovine rennet whey in bovine milk and in milk of other species and sources. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5171BRW |
EuroProxima Plus Cheese Fraud |
EuroProxima Plus Cheese Fraud is a competitive enzyme immunoassay for screening of the presence of bovine milk in cheese of other species and sources. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5171BKCC |
EuroProxima Plus Lactoferrin |
EuroProxima Plus Lactoferrin is a competitive enzyme immunoassay for screening and quantitative analysis of lactoferrin in various matrices. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5091LFER |
ELISA-TEK™ Cooked Meat Species Kit |
Assay for the positive identification of species content (various) in cooked samples: ELISA-TEK™ Cooked Mixed Species Kit: customized (Art. No. 510601) ELISA-TEK™ Cooked Meat 3 Species Kit: beef, pork, poultry (Art. No. 510603) ELISA-TEK™ … En savoir plus |
96 determinations | 5106** |
ELISA-TEK™ Raw Meat Species Kit |
Assay for the positive identification of species content (various) in raw samples: ELISA-TEK™ Raw Mixed Species Kit: customized (Art. No. 510501) ELISA-TEK™ Raw 3 Species Kit: cow, pig, poultry (Art. No. 510503) ELISA-TEK™ Raw 4 Species Kit: … En savoir plus |
96 determinations | 5105** |
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
RIDASCREEN® T-2 / HT-2 Toxin |
RIDASCREEN® T-2 / HT-2 Toxin is a competitive enzyme immunoassay for the quantitative analysis of the sum of T-2 and HT-2 toxin in oats, corn (maize), barley and wheat. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R3805 |
RIDASCREEN® Zearalenon |
RIDASCREEN® Zearalenon is a competitive enzyme immunoassay for the quantitative analysis of zearalenone residues in cereals, feed, beer, serum and urine. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each). | R1401 |
RIDASCREEN® DON |
RIDASCREEN® DON is a competitive enzyme immunoassay for the quantitative analysis of DON in cereals, malt, feed, beer and wort. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R5906 |
RIDASCREEN® T-2 Toxin |
RIDASCREEN® T-2 Toxin is a competitive enzyme immunoassay for the quantitative determination of T-2 toxin in cereals and feed. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R3801 |
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
EuroProxima Trenbolone |
This competitive enzyme immunoassay is used for the quantitative determination of trenbolone in urine, liver and tissue. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081TRENBO |
EuroProxima Florfenicol-amine |
A competitive enzyme immunoassay for screening and quantitative analysis of florfenicol-amine in various matrices. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5091FLOA |
EuroProxima Neomycin |
A competitive enzyme immunoassay for screening and quantitative analysis of neomycin in milk, milk powder, tissue, honey, serum/plasma and urine samples. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5111NEO |
EuroProxima Oxytetracycline |
A competitive enzyme immunoassay for screening and quantitative analysis of oxytetracyclines in various matrices. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5091OTC |
EuroProxima Tylosin |
EuroProxima Tylosin is a competitive enzyme immunoassay for screening and quantitative analysis of tylosin in various matrices. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5151TYL |
EuroProxima Virginiamycin |
EuroProxima Virginiamycin is a competitive enzyme immunoassay for quantitative analysis of virginiamycin in various matrices. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5151VIG |
EuroProxima Fluoroquinolones |
EuroProxima Fluoroquinolones is a competitive enzyme immunoassay for screening and quantitative analysis of a broad range of fluoroquinolones in various matrices. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5101FLUQG |
EuroProxima Fluoroquinolones II |
EuroProxima Fluoroquinolones II is a competitive enzyme immunoassay for screening and quantitative analysis of fluoroquinolones in various matrices. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5101FLUQII |
EuroProxima Promazine, generic |
EuroProxima Promazine, generic is a competitive enzyme immunoassay for screening and quantitative analysis of several promazines in tissue, liver, urine and kidney. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5201PROM |
EuroProxima Malachite Green Total/Crystal Violet |
EuroProxima Malachite Green Total/Crystal Violet is a competitive enzyme immunoassay for quantitative analysis of malachite green, leucomalachite green, crystal violet and leucocrystal violet in fish and shrimp. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5161MGT |
EuroProxima Azaperone-Azaperol |
EuroProxima Azaperone-Azaperol is a competitive enzyme immunoassay for screening and quantitative analysis of azaperone/azaperol in various matrices. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5201AZA |
EuroProxima Carazolol |
EuroProxima Carazolol is a competitive enzyme immunoassay for screening and quantitative analysis of carazolol in various matrices. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5201CARA |
EuroProxima Corticosteroid |
This competitive enzyme immunoassay is used for the quantitative determination of corticosteroids in milk, urine, muscle, liver and feed. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081COR |
EuroProxima Progesterone |
This competitive enzyme immunoassay is used for the quantitative determination of progesterone in milk and serum. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081PROG |
EuroProxima Stanozolol |
This competitive enzyme immunoassay is used for the quantitative determination of stanozolol in urine and faeces. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081STAN |
EuroProxima Triamcinolone |
This competitive enzyme immunoassay is used for the quantitative determination of triamcinolone in urine. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081TRIA |
EuroProxima Saxitoxin |
EuroProxima Saxitoxin is a competitive enzyme immunoassay for the screening and quantitative analysis of the shellfish poison saxitoxin in various matrices. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5191SAXI |
EuroProxima Lincomycin |
A competitive enzyme immunoassay for screening and quantitative analysis of lincomycin in various matrices. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5151LIN |
EuroProxima Gentamicin |
A competitive enzyme immunoassay for screening and quantitative analysis of gentamicin in various matrices. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5111GEN |
EuroProxima Flumequine |
EuroProxima Flumequine is a competitive enzyme immunoassay for screening and quantitative analysis of flumequine in various matrices. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5101FLUM |
EuroProxima Erythromycin |
EuroProxima Erythromycin is a competitive enzyme immunoassay for screening and quantitative analysis of erythromycin in various matrices. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5151ERY |
EuroProxima Domoic Acid |
EuroProxima Domoic Acid is a competitive enzyme immunoassay for quantitative analysis of domoic acid in scallop, mussel and oyster samples. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5191DOMO |
EuroProxima Dimetridazole |
EuroProxima Dimetridazole is a competitive enzyme immunoassay for quantitative analysis of dimetridazole in various matrices. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5091DIME |
EuroProxima Colistin |
A competitive enzyme immunoassay for screening and quantitative analysis of colistin in various matrices. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5151COL |
EuroProxima Ivermectin |
EuroProxima Ivermectin is an unique competitive enzyme immunoassay (ELISA) for the detection of ivermectin in urine, tissue and milk samples. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5141IVER |
EuroProxima Diethylstilbestrol (DES) |
A competitive enzyme immunoassay for screening and quantitative analysis of diethylstilbestrol (DES) in various matrices. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081DES |
EuroProxima Penicillin |
A competitive enzyme immunoassay for screening and quantitative analysis of penicillins in various matrices. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5091PEN |
RIDASCREEN® Tetracyclin |
RIDASCREEN® Tetracyclin is a competitive enzyme immunoassay for the quantitative analysis of tetracyclins in milk, milk powder, cheese, butter, dairy products (curd, yoghurt (plain/with fruits), kefir, cream, sour cream) honey, meat, sausage, fish, … En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R3505 |
RIDASCREEN® Chloramphenicol |
RIDASCREEN® Chloramphenicol is a competitive enzyme immunoassay for the quantitative analysis of chloramphenicol in milk, milk powder and dairy products, honey and royal jelly, meat, fish, shrimp, eggs, urine (also chloramphenicol glucuronide), … En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R1511 |
RIDASCREEN® Sulfamethazin |
RIDASCREEN® Sulfamethazin is a competitive enzyme immunoassay for the quantitative determination of sulfamethazine in milk, meat, honey, liver, kidney, fish, shrimp and egg. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each). | R3011 |
RIDASCREEN® Streptomycin |
RIDASCREEN® Streptomycin is a competitive enzyme immunoassay for the quantitative analysis of streptomycin in milk and milk powder, honey, meat, liver, kidney, shrimp and apple juice. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 wells each). | R3104 |
RIDASCREEN® Bacitracin |
RIDASCREEN® Bacitracin is a competitive enzyme immunoassay for the quantitative analysis of bacitracin in milk, meat, eggs, feed and urine. En savoir plus |
Microtiter plate with 96 wells (12 strips with 8 removable wells each). | R2901 |