ELISA
Efficiënte immunoassays voor de voedingsanalyse
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Technologieën
ELISA
ELISA (Enzyme-Linked Immunosorbent Assay) is een op antilichamen gebaseerde testmethode. Deze wijdverbreide technologie is gevoelig, snel en betrouwbaar.
Met de ELISA-testkits, vertrouwt R-Biopharm op een volop beproefde analytische methode die voldoet aan de vereisten van moderne laboratoria. RIDASCREEN® testen vertonen een hoge gevoeligheid en worden gekenmerkt door robuustheid. Voor de tijdgevoelige screening van diverse producten zijn RIDASCREEN®FAST testen verkrijgbaar. Deze testen maken het mogelijk om voedings- en diervoedermonsters binnen maximaal 30 min. te analyseren, inclusief monstervoorbereiding.
ELISA-principe
Antilichamen zijn eiwitten die in plasmacellen van gewervelde dieren worden geproduceerd als onderdeel van het adaptieve immuunsysteem tegen structuren (antigenen) die als lichaamsvreemd worden herkend. Antilichamen binden aan hun antigenen door een speciaal patroon van ionen- en hydrofobe interacties, waterstofbruggen en vanderwaalskrachten. De interactie tussen het antilichaam en zijn antigeen is selectief en zeer specifiek, vergelijkbaar met een slot en zijn sleutel.
De ELISA (Enzyme-Linked Immunosorbent Assay) is gebaseerd op deze selectieve en specifieke antilichaam-antigeenherkenning. Er zijn veel types van kwalitatieve of kwantitatieve ELISA-testen ontwikkeld. Voor de uitvoering van een ELISA-test is ten minste één specifiek antilichaam voor een bepaald antigeen nodig. Een hoofdprincipe is dat een van deze immunologische componenten wordt geïmmobiliseerd aan een vaste fase, de cupjes van een microtiterplaat. Er vindt interactie plaats tussen de analyt van het monster en het antilichaam-antigeensysteem. Deze interactie kan worden gevisualiseerd door enzymen, gekoppeld aan secundaire antilichamen of antigenen, en geeft aan of zich een antilichaam aan een antigeen heeft gebonden. Een toegevoegd substraat wordt door het gekoppelde enzym omgezet met kleurverandering als gevolg. Deze kan worden gemeten met een spectrofotometer.
Sandwich-ELISA en competitieve ELISA
Terwijl een sandwich-ELISA alleen intacte eiwitten en grote fragmenten met ten minste twee bindingsplaatsen detecteert, kan een competitieve ELISA grote en kleine eiwitfragmenten met maar één bindingsplaats detecteren. Om deze reden kan de competitieve ELISA ook voor sterk bewerkte voedingsmiddelen worden gebruikt.
Goede ELISA-praktijk
De handleiding over goede ELISA-praktijk biedt een uitgebreid overzicht voor zowel beginners als gevorderde analisten om de kwaliteit van de uitgevoerde ELISA-analyse te verbeteren. De handleiding biedt richtlijnen voor de realisatie van redelijke systeem- en gebruikscondities, waaraan moet worden voldaan voor optimale prestaties bij gebruik van de ELISA-testkits van R-Biopharm.
Product portfolio
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
RIDASCREEN® T-2 / HT-2 Toxin |
RIDASCREEN® T-2 / HT-2 Toxin is a competitive enzyme immunoassay for the quantitative analysis of the sum of T-2 and HT-2 toxin in oats, corn (maize), barley and wheat. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R3805 |
RIDASCREEN® Zearalenon |
RIDASCREEN® Zearalenon is a competitive enzyme immunoassay for the quantitative analysis of zearalenone residues in cereals, feed, beer, serum and urine. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | R1401 |
RIDASCREEN® DON |
RIDASCREEN® DON is a competitive enzyme immunoassay for the quantitative analysis of DON in cereals, malt, feed, beer and wort. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R5906 |
RIDASCREEN® T-2 Toxin |
RIDASCREEN® T-2 Toxin is a competitive enzyme immunoassay for the quantitative determination of T-2 toxin in cereals and feed. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R3801 |
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
EuroProxima Trenbolone |
This competitive enzyme immunoassay is used for the quantitative determination of trenbolone in urine, liver and tissue. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081TRENBO |
EuroProxima Florfenicol-amine |
A competitive enzyme immunoassay for screening and quantitative analysis of florfenicol-amine in various matrices. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5091FLOA |
EuroProxima Neomycin |
A competitive enzyme immunoassay for screening and quantitative analysis of neomycin in milk, milk powder, tissue, honey, serum/plasma and urine samples. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5111NEO |
EuroProxima Oxytetracycline |
A competitive enzyme immunoassay for screening and quantitative analysis of oxytetracyclines in various matrices. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5091OTC |
EuroProxima Tylosin |
EuroProxima Tylosin is a competitive enzyme immunoassay for screening and quantitative analysis of tylosin in various matrices. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5151TYL |
EuroProxima Virginiamycin |
EuroProxima Virginiamycin is a competitive enzyme immunoassay for quantitative analysis of virginiamycin in various matrices. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5151VIG |
EuroProxima Fluoroquinolones |
EuroProxima Fluoroquinolones is a competitive enzyme immunoassay for screening and quantitative analysis of a broad range of fluoroquinolones in various matrices. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5101FLUQG |
EuroProxima Fluoroquinolones II |
EuroProxima Fluoroquinolones II is a competitive enzyme immunoassay for screening and quantitative analysis of fluoroquinolones in various matrices. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5101FLUQII |
EuroProxima Promazine, generic |
EuroProxima Promazine, generic is a competitive enzyme immunoassay for screening and quantitative analysis of several promazines in tissue, liver, urine and kidney. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5201PROM |
EuroProxima Malachite Green Total/Crystal Violet |
EuroProxima Malachite Green Total/Crystal Violet is a competitive enzyme immunoassay for quantitative analysis of malachite green, leucomalachite green, crystal violet and leucocrystal violet in fish and shrimp. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5161MGT |
EuroProxima Azaperone-Azaperol |
EuroProxima Azaperone-Azaperol is a competitive enzyme immunoassay for screening and quantitative analysis of azaperone/azaperol in various matrices. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5201AZA |
EuroProxima Carazolol |
EuroProxima Carazolol is a competitive enzyme immunoassay for screening and quantitative analysis of carazolol in various matrices. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5201CARA |
EuroProxima Corticosteroid |
This competitive enzyme immunoassay is used for the quantitative determination of corticosteroids in milk, urine, muscle, liver and feed. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081COR |
EuroProxima Progesterone |
This competitive enzyme immunoassay is used for the quantitative determination of progesterone in milk and serum. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081PROG |
EuroProxima Stanozolol |
This competitive enzyme immunoassay is used for the quantitative determination of stanozolol in urine and faeces. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081STAN |
EuroProxima Triamcinolone |
This competitive enzyme immunoassay is used for the quantitative determination of triamcinolone in urine. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081TRIA |
EuroProxima Saxitoxin |
EuroProxima Saxitoxin is a competitive enzyme immunoassay for the screening and quantitative analysis of the shellfish poison saxitoxin in various matrices. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5191SAXI |
EuroProxima Lincomycin |
A competitive enzyme immunoassay for screening and quantitative analysis of lincomycin in various matrices. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5151LIN |
EuroProxima Gentamicin |
A competitive enzyme immunoassay for screening and quantitative analysis of gentamicin in various matrices. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5111GEN |
EuroProxima Flumequine |
EuroProxima Flumequine is a competitive enzyme immunoassay for screening and quantitative analysis of flumequine in various matrices. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5101FLUM |
EuroProxima Erythromycin |
EuroProxima Erythromycin is a competitive enzyme immunoassay for screening and quantitative analysis of erythromycin in various matrices. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5151ERY |
EuroProxima Domoic Acid |
EuroProxima Domoic Acid is a competitive enzyme immunoassay for quantitative analysis of domoic acid in scallop, mussel and oyster samples. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5191DOMO |
EuroProxima Dimetridazole |
EuroProxima Dimetridazole is a competitive enzyme immunoassay for quantitative analysis of dimetridazole in various matrices. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5091DIME |
EuroProxima Colistin |
A competitive enzyme immunoassay for screening and quantitative analysis of colistin in various matrices. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5151COL |
EuroProxima Ivermectin |
EuroProxima Ivermectin is an unique competitive enzyme immunoassay (ELISA) for the detection of ivermectin in urine, tissue and milk samples. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5141IVER |
EuroProxima Diethylstilbestrol (DES) |
A competitive enzyme immunoassay for screening and quantitative analysis of diethylstilbestrol (DES) in various matrices. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081DES |
EuroProxima Penicillin |
A competitive enzyme immunoassay for screening and quantitative analysis of penicillins in various matrices. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5091PEN |
RIDASCREEN® Tetracyclin |
RIDASCREEN® Tetracyclin is a competitive enzyme immunoassay for the quantitative analysis of tetracyclins in milk, milk powder, cheese, butter, dairy products (curd, yoghurt (plain/with fruits), kefir, cream, sour cream) honey, meat, sausage, fish, … Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R3505 |
RIDASCREEN® Chloramphenicol |
RIDASCREEN® Chloramphenicol is a competitive enzyme immunoassay for the quantitative analysis of chloramphenicol in milk, milk powder and dairy products, honey and royal jelly, meat, fish, shrimp, eggs, urine (also chloramphenicol glucuronide), … Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R1511 |
RIDASCREEN® Sulfamethazin |
RIDASCREEN® Sulfamethazin is a competitive enzyme immunoassay for the quantitative determination of sulfamethazine in milk, meat, honey, liver, kidney, fish, shrimp and egg. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | R3011 |
RIDASCREEN® Streptomycin |
RIDASCREEN® Streptomycin is a competitive enzyme immunoassay for the quantitative analysis of streptomycin in milk and milk powder, honey, meat, liver, kidney, shrimp and apple juice. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | R3104 |
RIDASCREEN® Bacitracin |
RIDASCREEN® Bacitracin is a competitive enzyme immunoassay for the quantitative analysis of bacitracin in milk, meat, eggs, feed and urine. Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each). | R2901 |
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
RIDASCREEN® Egg |
RIDASCREEN® Egg is a sandwich enzyme immunoassay developed for the quantitative analysis of native and processed hen´s egg in foods. The following foods were examined as representatives of the group of products including baked goods, sweets, … Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R6411 |
RIDASCREEN®FAST Gliadin sensitive |
RIDASCREEN®FAST Gliadin sensitive is a sandwich enzyme immunoassay for the determination of prolamins from gluten containing cereals in as gluten-free declared food (cookies, rice flour, oat flour, sausages, quinoa flour, chocolate, millet flour). Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R7051 |
RIDASCREEN® Gliadin |
RIDASCREEN® Gliadin (Art. No. R7001) is a R5 sandwich enzyme immunoassay based on specific monoclonal antibody to celiac toxic amino acid prolamin sequences to determine gliadin as a measure of gluten in food. It is applicable for the quantitative … Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R7001 |
RIDASCREEN®FAST Cashew |
The RIDASCREEN®FAST Cashew is a sandwich enzyme immunoassay developed for the quantitative analysis of Cashew in food. Exemplarily for the food groups pastries and sweets have been tested during the assay’s development: cookies, chocolate and ice … Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R6872 |
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
EuroProxima Plus Bovine Rennet Whey |
EuroProxima Plus Bovine Rennet Whey is a competitive enzyme immunoassay for screening of the presence of bovine rennet whey in bovine milk and in milk of other species and sources. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5171BRW |
EuroProxima Plus Cheese Fraud |
EuroProxima Plus Cheese Fraud is a competitive enzyme immunoassay for screening of the presence of bovine milk in cheese of other species and sources. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5171BKCC |
EuroProxima Plus Lactoferrin |
EuroProxima Plus Lactoferrin is a competitive enzyme immunoassay for screening and quantitative analysis of lactoferrin in various matrices. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5091LFER |
ELISA-TEK™ Cooked Meat Species Kit |
Assay for the positive identification of species content (various) in cooked samples: ELISA-TEK™ Cooked Mixed Species Kit: customized (Art. No. 510601) ELISA-TEK™ Cooked Meat 3 Species Kit: beef, pork, poultry (Art. No. 510603) ELISA-TEK™ … Read more |
96 determinations | 5106** |
ELISA-TEK™ Raw Meat Species Kit |
Assay for the positive identification of species content (various) in raw samples: ELISA-TEK™ Raw Mixed Species Kit: customized (Art. No. 510501) ELISA-TEK™ Raw 3 Species Kit: cow, pig, poultry (Art. No. 510503) ELISA-TEK™ Raw 4 Species Kit: … Read more |
96 determinations | 5105** |