Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS for detection of sterigmatocystin in a wide range of commodities.
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxin the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxin is then released from the column following elution with solvent. The eluate is collected, evaporated and reconstituted prior to analysis by HPLC or LC-MS/MS.
- Innovative product, first immunoaffinity column on the market prior to legislation being introduced.
- Improved clean-up and concentration of toxin from complex food matrices resulting in reduced chromatography
interference and lower detection limits.
- The columns are suitable for testing a wide range of commodities.
|Art. No.||RBRP125/ RBRP125B|
|Test format||RBRP125: 10 columns (3 ml format),|
RBRP125B: 50 columns (3 ml format)
|Sample preparation||A representative sample should be obtained by following one of the officially recognised sampling procedures. It is recommended that a minimum of 1 kg of representative sample is finely ground and a portion (10 - 50 g dependent on method used) of this is removed and extracted.|
|Incubation time||60 seconds|
|LOD (Detection limit)||7.5 ng/ml (ppb)|
Food and feed.
|Available application notes|
Methods are available for additional commodities. Please contact your local R-Biopharm distributor for further information.
Sterigmatocystin in food and feed.
|Evaluation||It is recommended to run at least a 3 - 6 point calibration curve. In constructing a suitable curve the levels of the calibration standards should bracket or include the range of expected results. The diluted standard solutions should be prepared fresh on the day of analysis and used within a 24 hour period.|