The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform.
The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS. Aflatoxins are required to be derivatised when analysed by HPLC. The total extraction and clean-up time takes approximately 20 minutes to perform. The result is improved clean-up and concentration of the toxins from food and feed samples giving a much cleaner chromatogram and therefore providing more accurate and sensitive detection. The columns also have the added advantage that they can be automated for large scale analysis of samples.
- The columns give excellent recoveries and low % RSD.
- Capacity and limit of detection exceed international legislation and CEN / AOAC requirements.
- The columns are suitable for testing a wide range of commodities.
|Article Numbers||RBRDP04 / RBRP04|
|Test format||10 columns (1 ml format) (RBRDP04) , |
25 columns (1 ml format) (RBRP04)
|Incubation time||60 sec|
|LOD (Detection limit)||M1 0.05 ng/ml (ppt)|
Milk and milkpowder.
Aflatoxin M1 in milk and milkpowder.
|Evaluation||It is recommended to run at least a 3 - 6 point calibration curve. In constructing a suitable curve the levels of the calibration standards should bracket or include the range of expected results. The diluted standard solutions should be prepared fresh on the day of analysis and used within a 24 hour period.|
|Approvals||International Dairy Federation approval. The columns have also been assessed in a number of European and CEN collaborative trials.|