Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS system for detection of chloramphenicol in a wide range of commodities.
The columns contain a gel suspension of monoclonal antibody specific to the antibiotic of interest. The sample is diluted with buffer, vortexed (or placed on an orbital shaker), filtered and passed slowly through the immunoaffinity column. Any antibiotic which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the antibiotic is then released from the column following elution with solvent. The eluate is collected, evaporated and reconstituted prior to analysis by HPLC or LC-MS/MS.
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits.
|Art. No.||P300 / P300B|
|Test format||10 wide format immunoaffinity columns (P300).|
50 wide format immunoaffinity columns (P300B).
|Detection limit||HPLC: 20 ng/ml of chloramphenicol.|
LC-MS/MS: 0.02 ng/ml of chloramphenicol.