A competitive enzyme immunoassay for screening and quantitative analysis of Salinomycin and Naracin in various matrices.
Salinomycin and Naracin belong to the group of polyether antibiotics (ionophores) and are fermentation products of the fungi Streptomyces albus and S. Aureofaciens, respectively. The main therapeutic application of ionophores is the prevention and treatment of coccidiosis in poultry. Coccidiosis is a parasitic disease, caused by protozoa resident in the intestinal epithelium, which occurs wherever animals are housed in small areas that are contaminated with coccidial oocysts.
The Ionophore ELISA is a competitive enzyme immunoassay based on antibodies directed against salinomycin.
|Test format||Microtiter plate, 96 wells.|
|Sample preparation||For tissue and pet feed fast and efficient methods are included in the kit manual.|
|Incubation time||90 minutes|
|LOD (Detection limit)||Tissue 4 ppb|
Pet feed 20 ppb
Salinomycin 200%, Naracin 100%, Monensin < 1%, Lasolocid < 1%