A competitive enzyme immunoassay for screening of raw and heated cow’s and buffalo’s milk in the milk and cheese of other species and sources.
Due to the lower prices of cow’s and buffalo’s milk, fraudulent mixing with higher priced milk of other species and sources is economically attractive but illegal and dangerous for allergic consumers. Most of the available immunoassays lack the detection of buffalo’s milk and the possibility to detect heattreated bovine milk due to denaturation of the target protein(s). This ELISA is based on a mouse monoclonal antibody (Mab) raised against the bovine ĸ-casein. The Mab recognises a 5 amino acidscontaining epitope on the glycomacropeptide (GMP) part of bovine ĸ-casein which is absent on the ĸcasein of other milk producing species.
The Milk Fraud/Bovine ELISA (BKCM) is a competitive enzyme immunoassay based on antibodies directed against K-casein.
|Test format||Microtiter plate, 96 wells.|
|Sample preparation||For various milk products fast and efficient extraction methods are included in the kit manual.|
|Incubation time||90 minutes|
Cow’s κ-casein 100%, Buffalo’s κ-casein 100%, Other bovine caseins 0%, Proteins of other species 0%, Proteins of other sources 0%