EuroProxima T-2 HT-2 Toxin
Art. No.: 5121THT
EuroProxima T-2 HT-2 Toxin is a competitive enzyme immunoassay for quantitative analysis of T-2 and HT-2 toxins in cereals, baby food, baby porridge and rye.
T-2 toxin and its metabolite HT-2 toxin belong to the group of trichothecenes, a large group of mycotoxins produced mainly by Fusarium species. Both T-2 and HT-2 toxins can be found in crops such as corn, wheat, barley and oats and also in products thereof. These toxins are harmful to both humans and animals. Cats and pigs are among the most sensitive species. T-2 and HT-2 impair protein and DNA synthesis and interfere with both red and white blood cells formation in bone marrow which leads to a number of adverse effects. Due to these toxic effect the Panel on Contaminants in the Food Chain (CONTAM Panel) of the European Food Safety Authority has set the tolerable daily intake for the sum of both T-2 and HT-2 toxins at 100 ng/kg body weight. In the EU, according to the Commission Recommendation 2013/165/EU, Member States with involvement of food and feed business operators are required to monitor for the presence of both T-2 and HT-2 toxins in food and feed. The indicative limits for T-2 and HT-2 toxins have been also established based on the occurrence data. These are 100–1000 µg/kg for unprocessed cereals, 50–200 µg/kg for cereal grains for direct human consumption, 25–200 µg/kg for cereal products for human consumption, 15 µg/kg for infant food and 250–2000 µg/kg for feed. If the sample is found to be contaminated above these values it is required to perform an investigation in order to identify factors causing these high levels. A limit of detection of an analytical screening method for the sum of T-2 and HT-2 toxins should preferably not be higher than 25 µg/kg.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each).|
|Sample preparation||For mentioned matrices fast and efficient extraction methods are included in the kit description.|
|Incubation time||1 h 30 min|
|LOD (Detection Limit)||Cereals (12.0 μg/kg (ppb)), baby food (6.0 μg/kg), baby porridge (4.8 μg/kg), rye (8.9 μg/kg)|
Cereals, baby food, baby porridge and rye.
T-2 and HT-2 toxins in cereals, baby food, baby porridge and rye.
|Evaluation||Microtiter plate spectrophotometer (450 nm)|