Lumitester PD-30 with use of LuciPac®Pen allows rapid detection of food and microbial related residues on product contact surfaces as a monitoring tool for the effectiveness of implemented cleaning and disinfection. The quantitative detection of the intra- and extracellular Adenosine Triphosphates (ATP) as well as Adenosine Monophosphate (AMP) is carried out in the portable luminometer Lumitester PD-30.
The energy stored within the ATP molecule is released as light by splitting of ATP to AMP (Adenosine Monophosphate). The light can be measured quantitatively and corresponds with the ATP level. The reaction uses the enzyme luciferase from the well-known organism firefly:
Luciferin + Luciferase + ATP (Luciferin-Luciferase-AMP) + Pyrophosphate
(Luciferin-Luciferase-AMP) Oxyluciferin + Luciferase + CO2 + AMP+ Light
Via the integrated AMP-recycling system based on Pyruvate-Phosphate Dikinase (PPDK) AMP could be detected as well as ATP on the surfaces. Therefore the test is more sensitive as cleaning control then other ATP systems.
|Sample preparation||Without sample preparation|
|Incubation time||10 sec|
|External link I||Video: practical use of the Lumitester PD-30 + LuciPac Pen|