Intended use:

RIDASCREEN® Biotin is a competitive enzyme binding assay for the quantitative analysis of added biotin in vitaminized food and vitamin products. The sample preparation for milk and milk powder allows the determination of the total biotin content (added and natural biotin).

For the detection of Biotin in serum samples a special kit is available under Art. No.: H1601.

General Information:

Biotin, also known as vitamin H, is an important nutritional factor and feed additive, playing a major role in the metabolism of both humans and animals alike. Biotin acts as a coenzyme and participates in the process of gluconeogenesis, biosynthesis of fatty acids and the metabolism of amino acids. Biotin deficiency in mammals leads to growth inhibition, loss of appetite and reduced utilization of food components. Advanced stages of avitaminosis manifest themselves in hair loss, dermatitis and signs of paralysis. The biotin requirement for adults is between 30 and 60 µg per day.

Art. No. R2201
Test format Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Sample preparation homogenization, (optional digestion with a-amylase, depending on sample matrices) heating, centrifugation, (optional Ascorbase-treatment) and dilution
Incubation time 1 h 30 min
Detection limit milk: approx. 5 µg/l (ppb)
milk powder: approx. 55 µg/kg (ppb)
grain products: approx. 3.7 µg/kg (ppb)
vitamin powder etc.: approx. 370 ng/kg (ppt)
vitamin juice: approx. 370 ng/l (ppt)

(corresponding to the standard substance)
Cross reactivity

Biotin: 100 %
Biotin-4-Amidobenzoat: approx. 89 %
Biocytin: approx. 83 %

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