RIDASCREEN® Melengestrolacetat is a competitive enzyme immunoassay for the quantitative analysis of melengestrol acetate in bovine perirenal fat and muscle meat. The performance of the test is adjusted to the current legal demands and well suited for the control of meat exported by the USA and Canada.
Synthetic gestagens can be used for estrus inhibition or synchronisation after break off and improvement of fertility. In addition one of these substances, melengestrol acetate (MLGA), is a licensed growth promoting feed additive for heifers in the USA and Canada. The admitted dose is 0.5 mg per day and head, given as a feed premix. Its mode of anabolic action is unclear, but it stimulates the ovarian synthesis of the endogenous anabolic steroid estradiol and that may have androgenic side effects. MLGA belongs to the most active synthetic gestagens. Its oral bioactivity is about 10 or 100 times higher than the activities of the gestagens chlormadinone acetate (CMA) or medroxyprogesterone acetate (MPA). If given parenterally the hormonal activity of MLGA is still 125 times higher than the one of progesterone. Due to its strongly lipophilic properties, MLGA is accumulated in fat 200-fold higher than in blood plasma.
In the European Union the use of sexual hormones for growth promoting purposes is generally forbidden since 1988, and also the import of meat from hormone treated cattle into the EU is prohibited.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||Extraction over night. Centrifugation, evaporation, degreasing, centrifugation and chromatographic purification with RIDA® C18 columns (Art. No. R2002).|
|Incubation time||2 h 30 min|
|LOD (Detection limit)||Bovine perirenal fat 0.3 μg/kg (ppb), muscle meat 0.075 μg/kg|
Bovine perirenal fat and muscle meat.
Melengestrol acetate in bovine perirenal fat and muscle meat.
|Evaluation||Microtiter plate spectrophotometer (450 nm)|