Intended use:

RIDASCREEN® Melengestrolacetat is a competitive enzyme immunoassay for the quantitative analysis of melengestrol acetate in bovine perirenal fat and muscle meat. The performance of the test is adjusted to the current legal demands and well suited for the control of meat exported by the USA and Canada.

General information:

Synthetic gestagens can be used for estrus inhibition or synchronisation after break off and improvement of fertility. In addition one of these substances, melengestrol acetate (MLGA), is a licensed growth promoting feed additive for heifers in the USA and Canada. The admitted dose is 0.5 mg per day and head, given as a feed premix. Its mode of anabolic action is unclear, but it stimulates the ovarian synthesis of the endogenous anabolic steroid estradiol and that may have androgenic side effects. MLGA belongs to the most active synthetic gestagens. Its oral bioactivity is about 10 or 100 times higher than the activities of the gestagens chlormadinone acetate (CMA) or medroxyprogesterone acetate (MPA). If given parenterally the hormonal activity of MLGA is still 125 times higher than the one of progesterone. Due to its strongly lipophilic properties, MLGA is accumulated in fat 200-fold higher than in blood plasma.

In the European Union the use of sexual hormones for growth promoting purposes is generally forbidden since 1988, and also the import of meat from hormone treated cattle into the EU is prohibited.

Accessories:

Specifications
Art. No. R6502
Test format Microtiter plate with 96 wells (12 strips with 8 wells each)
Sample preparation Extraction over night. Centrifugation, evaporation, degreasing, centrifugation and chromatographic purification with RIDA® C18 columns (Art. No. R2002).
Incubation time 2 h 30 min
LOD (Detection limit) Bovine perirenal fat 0.3 μg/kg (ppb), muscle meat 0.075 μg/kg
Validated matrices

Bovine perirenal fat and muscle meat.

Detected analyte

Melengestrol acetate in bovine perirenal fat and muscle meat.

Evaluation Microtiter plate spectrophotometer (450 nm)
E-mail linksales@r-biopharm.de
Files
InstructionsGerman/English
SDSr6502_german.zip (German)
r6502_english-1.zip (English)
r6502_french.zip (French)
r6502_spanish.zip (Spanish)
r6502_italian.zip (Italian)
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