RIDASCREEN®FAST Peanut test is a sandwich enzyme immunoassay for the quantitative analysis of peanuts or parts of peanuts in food. Exemplarily for different food groups, breakfast cereals, cookies, ice cream, and milk chocolate have been approved. The assay can be likely used for analysis of other food samples too – this has to be checked by the user. Peanut can be present as an ingredient or as a contamination in raw or heated food.
Although peanut (Arachis hypogaea) belongs to the legumes and not to the nuts, its taste and its usual consumption is similar to tree nuts. So to speak, peanut is one of the most popular “nuts” worldwide. Peanuts are very common ingredients of chocolate products and are also consumed as snacks and in bakery products such as cookies.
Peanut is also one of the five foods, which are the most frequent cause of food allergies. Allergies against peanuts are among the most severe allergies and can lead to life-threatening anaphylactic shocks. Consumption of probably less than a milligram of peanut can induce allergic reactions in very sensitized individuals. Therefore, labelling of peanut containing products is very important and compulsory in the USA and the European Union as well as in many other countries. The main threat for sensitized individuals is contamination of peanut in food which is intended to be peanut-free (e.g. peanut traces in chocolate which should not contain peanuts). Therefore, it is important to test foods which may have been contaminated during their production process for the presence of unintended traces of peanuts.
The allergen can be present as an ingredient or as a contamination in raw and cooked products. According to the regulation (EU) No. 1169/2011, peanut and products thereof must be declared on food labels. Similar regulations exist e.g. in the USA, Canada, Australia and New Zealand. RIDASCREEN®FAST Peanut was validated with breakfast cereals, cookies, ice cream, and milk chocolate in an independent study by three independent US laboratories under the supervision of the AOAC Research Institute. The study resulted in a specifity of 99.2 % (238/240) and a sensitivity of 100 % (240/240).
The ELISA RIDASCREEN®FAST Peanut was approved by the AOAC Performance Tested MethodsSM Program and was assigned PTM Certification No. 030404 by the AOAC Research Institute. The in-house validation data was confirmed by an independent lab. The sandwich ELISA is applicable for the detection of peanut in cookies, ice cream, breakfast cereal and milk chocolate.
- AOAC-RI 030404 validated method.
- Widespread testing for cross reactivity (more than 80 matrices have been evaluated).
- Validation with breakfast cereals, cookies, ice cream and milk chocolate in an independent study by 3 independent US laboratories under the supervision of AOAC Research Institute.
o Specificity: 99.2 % (238/240)
o Sensitivity: 100 % (240/240)
|Test format||Microtiter plate with 48 wells (6 strips with 8 removable wells each)|
|Sample preparation||Homogenization, extraction and centrifugation|
|Incubation time||30 min|
|LOD (Detection limit)||0.13 mg / kg (ppm) peanut (mean)|
0.04 – 0.19 mg / kg (ppm) peanut*
0.03 mg / kg (ppm) peanut protein
*depending on matrix
|LOQ (Limit of quantification)|
2.5 – 20 mg / kg (ppm) peanut
There is a cross reactivity to green pea, lentils, wheat semolina and fenugreek.
Representative for different food groups: breakfast cereals, cookies, ice cream, and milk chocolate.
|Available application notes|
Peanut proteins (incl. the peanut allergen Ara h 1 and Ara h 2)
|Evaluation||Microtiter plate spectrophotometer (450 nm)|
|External link I||RIDASCREEN® FAST PEANUT, a rapid and safe tool to determine peanut contamination in food|