RIDA®STAMP Coliform is an agar for the detection of coliform bacteria on surfaces. The RIDA®STAMP plates consist of a special 10 cm2 petri dish containing organism specific agar which is protruding above the surface of the plate in order to allow direct contact with the selected flat surface or solid food sample.
Regular sampling on cleaned and sanitised surfaces will lead to a complete quantitative hygiene monitoring process. Repeated controls will play a part in contributing to good workplace hygiene according to the concepts of HACCP and support GMP guidelines.
The term “coliform bacteria” doesn’t describe a taxonomical group beyond the eubacteria. It actually is more a summary of some genera from the family of enterobacteria. Their common biochemical characteristics are a positive lactose reaction as well as a negative oxidase reaction. These bacteria are easily to identify using nutrient media which contain chromogenic substrates for their enzyme β-galactosidase (e.g.: X-GAL).
Coliform bacteria are considered to be indicators for faecal contaminations. Detection of coliform bacteria on surfaces in the production environment or solid foods indicates that the hygienic conditions in the food production process need to be optimised.
The agar medium of RIDA®STAMP Coliform contains X-GAL. Thus, colonies of coliform bacteria show a characteristic blue or blue-green colouration while growing on the agar surface. Other bacterial species are not able to grow or their colonies are of different colour.
All grown blue or blue-green colonies have to be counted to determine the number of total coliform bacteria.
|Art. No.||HS0411, HS0412|
|Test format||25 or 50 agar plates, respectively, five plates are packed in one plastic bag|
|Sample preparation||not required|
|Incubation time||24 - 48 hours at 36 ± 1 °C|
|Detection limit||1 cfu/10 cm2|