The kit is intended to be used for the fast and simple isolation and detection of Histomonas meleagridis – DNA from tissue samples.
Each reaction contains an internal amplification control (IAC).
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche LightCycler® 2.0, Applied Biosystems 7500, Qiagen Rotor-Gene Q and R-Biopharm RIDA®CYCLER.
|Test format||100 reactions|
|Sample preparation||Lysis buffer is in the kit included.|
|LOD (Detection limit)||The SureFast® Histomonas meleagridis ONE real-time PCR has a limit of detection of 5 DNA copies.|