The test is a real-time RT-PCR for the direct and qualitative detection of influenza A. The test contains an Internal Control RNA (ICR, consisting of MS2-bacteriophage) as an internal control of sample preparation procedure and to determine possible PCR-inhibition.
For RNA-preparation the use of SureFast® PREP DNA/RNA Virus is recommended.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm (FAM and VIC/HEX) at the same time.
|Test format||100 reactions|
|LOD (Detection limit)||The SureFast® Influenza A PLUS real-time RT-PCR has a limit of detection of 25 RNA copies.|