The SureFast® STEC 4plex is a real-time PCR for for the direct, qualitative detection and differentiation of DNA sequences of the Escherichia coli virulence factors stx1 (subtype a-d), stx2 (subtype a-g), eae and the Escherichia coli Serotype O157.
Each reaction contains an internal amplification control (PLUS).
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of four fluorescence emissions at 522 nm, 553 nm, 610 nm and 670 nm (FAM, VIC, ROX and Cy5) at the same time.
The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, BioRad CFX 96, Roche LightCycler® 480 II/ Roche cobas z 480 Analyzer 2, BMS MIC, Qiagen Rotorgene and Applied Biosystems 7500.
|Test format||100 reactions|
|Detection limit||The SureFast® Vibrio 4plex real-time PCR has a limit of detection of < 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|