SureFood® ALLERGEN 4plex Macadamia / Brazil Nut / Pecan + IAC
Art. No.: S3403
Product info
Intended use
The SureFood® ALLERGEN 4plex Macadamia/Brazil Nut/Pecan + IAC is a real-time PCR for the direct, qualitative detection and differentiation of specific macadamia (Macadamia ternifolia), brazil nut (Bertholletia excelsa) and pecan (Carya illinoinensis) DNA sequences according to directive (EC) 1169/2011.
General Information
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Aria Dx, Bio-Rad CFX96 Dx, Roche LightCycler® 480 II and R-Biopharm RIDACYCLER.
Accessories
Specifications | |
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Art. No | S3403 |
Test format | 100 reactions |
Sample preparation | For DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 is recommended. |
LOD (Detection Limit) | The SureFood® ALLERGEN 4plex Macadamia/Brazil Nut/Pecan + IAC PCR has a limit of detection of ≤ 0.4 mg / kg using SureFood® PREP Advanced, protocol 1. |
Cross Reactivity. | For the Pecan real-time PCR system cross reactivity was observed with DNA extracts from shagbark hickory (Carya Ovata). |