SureFood® ALLERGEN Fish
Art. No.: S3610
Product info
Intended use
The SureFood® ALLERGEN Fish is a real-time PCR for the direct, qualitative and / or quantitative detection of specific fish DNA sequences according to directive (EC) 1169/2011. The group of fishes contains about 30000 species. Due to the large number of different species a practical verification of all is impossible. More detailed information is available in the Validation Report.
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (Art. No. S3301) containing 40 mg fish / kg food sample and a detection system including a standard series is required.
General Information
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical verification of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Bio-Rad CFX96 Dx, R-Biopharm RIDACYCLER and Agilent AriaDx.
Accessories
Dear customers,
we have started to provide the documents for our products in an electronic format. These are the Instructions for Use (IFU), the Safety Data Sheets (SDS) and the Certificate of Analysis (CoA). For batches placed on the market after 01 January 2023, you can find our documents on the eIFU portal eifu.r-biopharm.com.
| Specifications | |
|---|---|
| Art. No | S3610 |
| Test format | 100 reactions |
| Sample preparation | For DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended. |
| LOD (Detection Limit) | The SureFood® ALLERGEN Fish PCR has a limit of detection of ≤ 1 mg / kg using SureFood® PREP Advanced, protocol 1. |
| LOQ (Limit of quantification | 4 mg / kg using SureFood® PREP Advanced, protocol 1 |
| Cross Reactivity. | Cross reactivity was observed with DNA extracts from muscovy duck (Cairina moschata). |
