Intended use:

This will be replaced by S3605 in January 2018! Further information.

 

The test detects celery DNA according to directive (EC) 1169/2011. For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 containing 40 mg celery seeds / kg food sample and a standard dilution is required. The real-time PCR assay can be used with established real-time PCR instruments (Roche LightCycler®, Rotor-Gene Q, ABI PRISM, Eppendorf realplex, BioRad CFX96, Agilent MxSeries etc.).

General Information:

The test detects the quantitative DNA amount of celery in food samples according to EU regulation 2007/68 EC. For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 containing 40 mg celery seeds / kg food sample and a standard dilution is recommended. The real-time PCR assay can be used with established real-time PCR instruments.

For DNA-preparation the use of SureFood® PREP Advanced, protocol 1 is recommended.

Specifications
Art. No. S3205
Detection limit The SureFood® ALLERGEN QUANT Celery PCR has a limit of detection of ≤ 0.4 mg/kg. The limit of
quantification is 1 mg/kg using SureFood® PREP Advanced, protocol 1. The limit of detection and limit of
quantification were determined using the SureFood® QUANTARD Allergen 40 (matrix: corn flour).
The limit of detection of the complete quantitative determination (DNA extraction and real-time PCR)
depends on sample matrix, processing grade, DNA-preparation and DNA-content.
Files
InstructionsGerman/English
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