The SureFood® ANIMAL ID 3plex Rat/Mouse+IAAC is a real-time PCR kit for the direct, qualitative detection and differentiation of specific Brown rat (Rattus norvegicus), Black rat (Rattus rattus) and House mouse (Mus musculus) DNA sequences.
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of three fluorescence emissions at the channels FAM, VIC/HEX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, BioRad CFX 96 Dx, R-Biopharm RIDA®CYCLER, Roche LightCycler® 480 II and Qiagen Rotor-Gene Q.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended.|
|LOD (Detection limit)||The SureFood® ANIMAL ID 3plex Rat/Mouse+IAAC real-time PCR is developed for the detection of rat and mouse DNA in muscle meat mixture at a relative amount of < 500 DNA copies.|
Cross reactivity was observed with DNA extracts from Turkestan rat (Rattus pyctoris), Asian house rat (Rattus tanezumi) and Algerian mouse (Mus spretus).