SureFood® ANIMAL ID 3plex Rat/Mouse+IAAC

Art. No.: S6127

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Product info

SureFood® ANIMAL ID 3plex Rat/Mouse+IAAC

Art. No.: S6127

Intended use

The SureFood® ANIMAL ID 3plex Rat/Mouse+IAAC is a real-time PCR kit for the direct, qualitative detection and differentiation of specific Brown rat (Rattus norvegicus), Black rat (Rattus rattus) and House mouse (Mus musculus) DNA sequences.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of three fluorescence emissions at the channels FAM, VIC/HEX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, BioRad CFX 96 Dx, R-Biopharm RIDA®CYCLER, Roche LightCycler® 480 II and Qiagen Rotor-Gene Q.

Accessories

Specifications
Art. NoS6127
Test format100 reactions
Sample preparationFor DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended.
LOD (Detection Limit)The SureFood® ANIMAL ID 3plex Rat/Mouse+IAAC real-time PCR is developed for the detection of rat and mouse DNA in muscle meat mixture at a relative amount of < 500 DNA copies.
Cross Reactivity.

Cross reactivity was observed with DNA extracts from Turkestan rat (Rattus pyctoris), Asian house rat (Rattus tanezumi) and Algerian mouse (Mus spretus).

Files
InstructionsGerman/English