The SureFood® ANIMAL ID 4plex Bison/Water Buffalo/Kangaroo+IAAC is a real-time PCR for the direct, qualitative detection and differentiation of specific bison (Bison spp.), water buffalo (Bubalus spp.) and kangaroo (Macropodidae) DNA sequences.
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER and Roche LightCycler® 480 II.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended.|
|LOD (Detection limit)||The SureFood® ANIMAL ID 4plex Bison/Water Buffalo/Kangaroo +IAAC real-time PCR is developed for the detection of bison, water buffalo and kangaroo DNA in muscle meat mixture at a relative amount of 0.5 %.|
Cross reactivity was observed with DNA extracts from yak (Bos grunniens) with < 0.1 %.