The test detects camel DNA of Arabian camel (Camelus dromedarius) and of Bactrian camel (Camelus ferus).
Each reaction contains an internal amplification control and an internal detection assay for vertebrates DNA (IAAC). The
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 510 nm and 580 nm at the same time. The technical validation of instruments was performed on Roche LightCycler® 2.0*, Roche LightCycler® 480 II, Roche cobas z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biossystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, Agilent AriaDx and Agilent Mx3005P.
*For the use of the Roche LightCycler® 2.0 a Color Compensation is necessary. The SureCC Color Compensation Kit II (Art. No. F4010) must be used for the color compensation of such devices.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.|
|Detection limit||The SureFood® ANIMAL ID Camel IAAC real-time PCR is developed for the detection of camel DNA in muscle meat mixture at a relative amount of 0.1 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|