The test detects water buffalo (Bubalus arnee) DNA. Each reaction contains an internal amplification control and an internal detection assay for vertebrates DNA (IAAC).
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 510 nm and 580 nm at the same time. The technical validation of instruments was performed on Roche LightCycler® 2.0*, Roche LightCycler® 480 II, Roche cobas z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biossystems 7500, Bio-Rad CFX96, LTF MyGo Pro, Agilent AriaDx and Agilent Mx3005P.
*note: A Color Compensation is necessary for users of the Roche LightCycler® 2.0. The SureCC Color Compensation kit II (Art. No. F4010) must be used for the color compensation of such devices.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.|
|LOD (Detection limit)||The SureFood® ANIMAL ID Waterbuffalo IAAC real-time PCR is developed for the detection of waterbuffalo DNA in muscle meat mixture at a relative amount of ≥0.1 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|