The test detects the relative quantitative equine DNA. The family of Equidae contains horse, donkey and zebra. The kit contains two PCR systems, one for detection of an equine specific gene (Equus detection gene) and one for the detection of an animal gene (Ref reference gene).
The real-time PCR assay can be used with established real-time PCR instruments (Roche LightCycler®, Rotor-Gene Q, ABI PRISM, Eppendorf realplex, BioRad CFX96, Agilent MxSeries etc.).
|Test format||2x 50 reactions|
|Sample preparation||For DNA-preparation the use of SureFood® PREP Basic kit is recommended.|
|Detection limit||The equine specific PCR has a limit of detection of < 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content.|
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of the reference gene are present, the relative quantitation limit for equine DNA is 0.1 %.