Intended use:

The real-time PCR test detects DNA of buckwheat (Fagopyrum esculentum) qualitatively. Each reaction contains an internal amplification control (IAC).

General information:

For DNA preparation, the use of SureFood® PREP Advanced (S1053), protocol 1, is recommended.

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Applied Biosystems 7500, Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche LightCycler® 2.0, Roche cobas z 480 Analyzer and Qiagen Rotor-Gene Q.

The SureFood® PCR systems are very sensitive and therefore even a small amount of target DNA is sufficient for a successful analysis.

Accessories:

Specifications
Art. No. S7005
Test format 100 reactions
Sample preparation For DNA-preparation the use of SureFood® PREP Advanced, protocol 1, is recommended.
LOD (Detection limit) ≤ 0.4 mg / kg
Evaluation Real-time PCR instrument with two detection channels (510 nm and 580 nm)
E-mail linksales@r-biopharm.de
Files
InstructionsGerman/English
SDSerklaerung-zu-congen-msds_2018-12.pdf (German)
confirmation-congen-msds_2018-12.pdf (English)
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qtpydb-0048_gen-ial-test