The test detects buckwheat DNA (Fagopyrum esculentum). Each reaction contains an internal amplification control.
The real-time PCR assay can be used with established real-time PCR instruments equipped for detection of two fluorescence emissions at 510 nm and 580 nm (FAM and VIC/HEX) at the same time. The technical validation of instruments was performed on Applied Biosystems 7500, Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q and LTF MyGo Pro.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.|
|Detection limit||The SureFood® Buckwheat real-time PCR has a limit of detection of <= 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|