The real-time PCR test detects DNA of buckwheat (Fagopyrum esculentum) qualitatively. Each reaction contains an internal amplification control (IAC).
For DNA preparation, the use of SureFood® PREP Advanced (S1053), protocol 1, is recommended.
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Applied Biosystems 7500, Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche LightCycler® 2.0, Roche cobas z 480 Analyzer and Qiagen Rotor-Gene Q.
The SureFood® PCR systems are very sensitive and therefore even a small amount of target DNA is sufficient for a successful analysis.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation the use of SureFood® PREP Advanced, protocol 1, is recommended.|
|LOD (Detection limit)||≤ 0.4 mg / kg|
|Evaluation||Real-time PCR instrument with two detection channels (510 nm and 580 nm)|