The test detects Alaska pollock (Gadus chalcogrammus or Theragra chalcogramma) DNA. Each reaction contains an internal amplification control and an internal detection assay for animal DNA (IAAC). The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm at the same time. For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.
|Test format||50 reactions|
|Detection limit||The SureFood® FISH ID Gadus chalcogrammus IAAC real-time PCR is developed for the detection of rainbow trout DNA in muscle meat mixture at a relative amount of 5 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|