The test detects skipjack (Katsuwonus pelamis) DNA. Each reaction contains an internal amplification control and an internal detection assay for vertebrates DNA (IAAC).
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm at the same time.
|Test format||50 reactions|
|Sample preparation||For DNA-preparation the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.|
|LOD (Detection limit)||The SureFood® FISH ID Katsuwonus pelamis IAAC (R&D Version) real-time PCR is developed for the detection of skipjack in muscle meat mixture at a relative amount of 1 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|