The test detects atlantic salmon (Salmo salar) DNA.
Each reaction contains an internal amplification control and an internal detection assay for animal DNA (IAAC).
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm at the same time. The technical validation of instruments was performed on Roche LightCycle®r 2.0*, Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Applied Biossystems 7500, Bio-Rad CFX96 Agilent Mx3005P.
*note: A Color Compensation is necessary for users of the Roche LightCycler® 2.0. The SureCC Color Compensation kit II (Art. No. F4010) must be used for the color compensation of such devices.
|Test format||50 reactions|
|Detection limit||The SureFood® FISH ID Salmo salar IAAC real-time PCR is developed for the detection of atlantic salmon DNA in muscle meat mixture at a relative amount of 1 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|