The test detects the relative quantitative MON810 Corn DNA amount.
Therefore the kit contains two PCR systems, one specific for the MON810 Corn (OECD unique identifier MON-ØØ81Ø-6) and the other one specific for Corn (the reference gene). The event specific detection of MON810 Corn is according to EN ISO 21570 and the official method of the European Commission.
The real-time PCR assay can be used with established real-time PCR instruments. The technical validation of instruments was performed on Agilent Mx3005P, Agilent Aria Dx, BioRad CFX 96, Roche LightCycler® 480 II, Roche cobas Z 480 Analyzer, Roche LightCycler® 2.0, Applied Biosystems 7500, Qiagen Rotor-Gene Q and LTF MyGo Pro.
|Test format||2 x 50 reactions|
|Sample preparation||For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.|
|LOD (Detection limit)||The MON810 corn PCR has a limit of detection of ≤ 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content.|
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of corn specific reference gene are present, the relative quantitation limit for MON810 Corn DNA is 0.1 %.