Intended use:

The test detects the relative quantitative GA21-Corn DNA amount.

Therefore the kit contains two PCR systems, one specific for the GA21-Corn (OECD unique identifier MON-ØØØ21-9) and the other one specific for Corn (reference system). The event-specific detection of GA21-Corn is according to the validated method of the European Commission.

 

General information:

The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, BioRad CFX96, Roche LightCycler®480 II/ Roche cobas z Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500.

Accessories:

Specifications
Art. No. S2054
Test format 2 x 50 reactions
Sample preparation For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.
LOD (Detection limit) The GA21-Corn PCR has a limit of detection of ≤ 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content.
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of Corn specific reference gene are present, the relative quantitation limit for GA21-Corn DNA is 0.1 %.
Files
InstructionsGerman/English
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