The test detects the relative quantitative GA21-Corn DNA amount.
Therefore the kit contains two PCR systems, one specific for the GA21-Corn (OECD unique identifier MON-ØØØ21-9) and the other one specific for Corn (reference system). The event-specific detection of GA21-Corn is according to the validated method of the European Commission.
The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, BioRad CFX96, Roche LightCycler®480 II/ Roche cobas z Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500.
|Test format||2 x 50 reactions|
|Sample preparation||For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.|
|LOD (Detection limit)||The GA21-Corn PCR has a limit of detection of ≤ 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content.|
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of Corn specific reference gene are present, the relative quantitation limit for GA21-Corn DNA is 0.1 %.