Intended use:

The test detects the relative quantitative MON89034 Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the MON89034 Corn (OECD unique identifier MON-89Ø34-3) and the other one specific for Corn (reference system). The event specific MON89034 Corn detection is according to the validated method of the European Commission.

General information:

The technical validation of instruments was performed on Agilent Mx3005P, Agilent Aria Dx, Applied Biosystems 7500, BioRad CFX96, R-Biopharm RIDA®CYCLER, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer and Qiagen Rotor-Gene Q.

Accessories:

Specifications
Art. No. S2071
Test format 2 x 50 reactions
Sample preparation For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.
LOD (Detection limit) The MON89034 Corn PCR has a limit of detection of < 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content.
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of Corn specific reference gene are present, the relative quantitation limit for MON89034 Corn DNA is 0.1 %.
Files
InstructionsGerman/English
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qtpydb-0048_gen-ial-test