The test detects the relative quantitative MON89034 Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the MON89034 Corn (OECD unique identifier MON-89Ø34-3) and the other one specific for Corn (reference system). The event specific MON89034 Corn detection is according to the validated method of the European Commission.
The technical validation of instruments was performed on Agilent Mx3005P, Agilent Aria Dx, Applied Biosystems 7500, BioRad CFX96, R-Biopharm RIDA®CYCLER, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer and Qiagen Rotor-Gene Q.
|Test format||2 x 50 reactions|
|Sample preparation||For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.|
|LOD (Detection limit)||The MON89034 Corn PCR has a limit of detection of < 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content.|
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of Corn specific reference gene are present, the relative quantitation limit for MON89034 Corn DNA is 0.1 %.