The test detects the relative NK603 Corn DNA amount to the total corn DNA content. Therefore the kit contains two PCR systems, one for detection of a NK603 Corn specific gene (OECD unique identifier MON-ØØ6Ø3-6) and one for the detection of a corn gene (reference gene).
The specific NK603 Corn detection is according to the official and validated method of the European Commission.
The real-time PCR assay can be used with established real-time PCR instruments. The technical validation of instruments was performed on Agilent Mx3005P, Agilent Aria Dx, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer and Qiagen Rotor-Gene Q.
|Test format||2x50 reacions|
|Sample preparation||For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.|
|LOD (Detection limit)||The NK603 Corn PCR has a limit of detection of ≤ 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content.|
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of corn specific reference gene are present, the relative quantitation limit for NK603 Corn DNA is 0.1 %.