The SureFood® Rice is a real-time PCR for the direct, qualitative detection of a specific rice (Oryza sativa) DNA sequence.
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Agilent Aria Dx, Applied Biosystems 7500, Bio-Rad CFX96, Roche LightCycler® 480 II, Qiagen RotorGene Q, LTF MyGo Pro and R-Biopharm RIDA®CYCLER.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.|
|LOD (Detection limit)||The SureFood® Rice real-time PCR has a limit of detection of < 5 DNA copies and 0.01 % respectively.|