{"id":340839,"date":"2024-08-22T04:11:08","date_gmt":"2024-08-22T02:11:08","guid":{"rendered":"https:\/\/food.r-biopharm.com\/?page_id=340839"},"modified":"2025-10-07T09:44:10","modified_gmt":"2025-10-07T07:44:10","slug":"pcr-em-tempo-real","status":"publish","type":"page","link":"https:\/\/food.r-biopharm.com\/pt-br\/tecnologias\/pcr-em-tempo-real\/","title":{"rendered":"PCR em Tempo Real"},"content":{"rendered":"<!-- hero image from block -->\n<div class=\"acf-block block-hero term-slug-- term- \">\n\n    <img decoding=\"async\" class=\"block-hero__image\" src=\"https:\/\/food.r-biopharm.com\/wp-content\/uploads\/2021-11_PCR_3000x695.jpg\">\n\n    \n        <div class=\"block-hero__textbox\">\n                             <!-- Default behavior for other pages -->\n                <h1>\n                    <span class=\"block-hero__textbox__headline\">\n                        PCR em Tempo Real                    <\/span>\n                    <br>\n                    <span class=\"block-hero__textbox__subline\">\n                        Diagn\u00f3sticos Moleculares na An\u00e1lise de Alimentos                    <\/span>\n                <\/h1>\n                    <\/div>\n\n    \n<\/div>\n\n\n\n\n\n<div class=\"acf-block block-taxonomy block-taxonomy__position--header\">\n\t\t\t<!-- Flexy Breadcrumb -->\n\t\t\t<nav class=\"fbc fbc-page\" aria-label=\"Breadcrumbs\">\n\n\t\t\t\t<!-- Breadcrumb wrapper -->\n\t\t\t\t<div class=\"fbc-wrap\">\n\n\t\t\t\t\t<!-- Ordered list-->\n\t\t\t\t\t<ol class=\"fbc-items\" itemscope itemtype=\"https:\/\/schema.org\/BreadcrumbList\">\n\t\t\t\t\t\t            <li itemprop=\"itemListElement\" itemscope itemtype=\"https:\/\/schema.org\/ListItem\" class=\"fbc-item fbc-item-home\">\n                <a itemprop=\"item\" href=\"https:\/\/food.r-biopharm.com\" title=\"Home\">\n                                        <span itemprop=\"name\">Home<\/span>\n                <\/a>\n                <meta itemprop=\"position\" content=\"1\" \/>\n            <\/li><li class=\"fbc-separator-item\" aria-hidden=\"true\"><span class=\"fbc-separator\">\/<\/span><\/li>\t\t\t\t\t<\/ol>\n\t\t\t\t\t\n<script type=\"application\/ld+json\" class=\"flexy-breadcrumb-schema\">{\"@context\":\"https:\/\/schema.org\",\"@type\":\"BreadcrumbList\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/food.r-biopharm.com\"}]}<\/script>\n\t\t\t\t\t<div class=\"clearfix\"><\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/nav>\n\t\t\t\n    <span class=\"block__title\">Tecnologias<\/span>            <div class=\"block__content\n            show-icons            \">\n                    <div class=\"swiper-container\">\n            <div role=\"navigation\" aria-label=\"Tecnologias navigation\" class=\"swiper-wrapper\">\n                            \n                <\/div>\n            <\/div>\n            <div class=\"swiper-button-prev swiper-button-prev__taxonomy\"><\/div>\n            <div class=\"swiper-button-next swiper-button-next__taxonomy\"><\/div>\n            <\/div>\n<\/div>\n\n\n\n<div class=\"acf-block row row--narrow background--\">\n        \n\n<div class=\"wp-block-column column column--75 is-layout-flow wp-block-column-is-layout-flow\">\n<h1 class=\"wp-block-heading\">PCR em Tempo Real<\/h1>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>A PCR (rea\u00e7\u00e3o em cadeia da polimerase) \u00e9 um m\u00e9todo anal\u00edtico importante tanto na an\u00e1lise de alimentos quanto em diagn\u00f3sticos m\u00e9dicos.<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Um teste de PCR em tempo real duplica e analisa sequ\u00eancias espec\u00edficas de DNA. Na an\u00e1lise de alimentos, os testes de PCR s\u00e3o utilizados em muitas aplica\u00e7\u00f5es, como a detec\u00e7\u00e3o de microrganismos patog\u00eanicos, identifica\u00e7\u00e3o de al\u00e9rgenos, detec\u00e7\u00e3o de organismos geneticamente modificados (OGM) ou identifica\u00e7\u00e3o de esp\u00e9cies animais. Os testes de PCR oferecem muitas vantagens: s\u00e3o muito espec\u00edficos, sens\u00edveis, robustos, r\u00e1pidos, confi\u00e1veis e, al\u00e9m disso, os testes podem ser automatizados.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Seguro: <\/strong>m\u00e9todo espec\u00edfico e validado<\/li>\n\n\n\n<li><strong>Confi\u00e1vel:<\/strong> kits de teste controlados por qualidade<\/li>\n\n\n\n<li><strong>F\u00e1cil de usar: <\/strong>prepara\u00e7\u00e3o universal de amostras<\/li>\n\n\n\n<li><strong>Eficaz: <\/strong>capaz de multiplexar e quantificar<\/li>\n\n\n\n<li><strong>Vers\u00e1til:<\/strong> muitas \u00e1reas de aplica\u00e7\u00e3o<\/li>\n<\/ul>\n\n\n\n<div class=\"block-featuredvideo row\">\n\n    \n        <div class=\"column column--50\">\n\n            <div class=\"iframe-wrapper\">\n                 <div class=\"brlbs-cmpnt-container brlbs-cmpnt-content-blocker brlbs-cmpnt-with-individual-styles\" data-borlabs-cookie-content-blocker-id=\"default\" data-borlabs-cookie-content=\"PGlmcmFtZSB0aXRsZT0iUmVhbHRpbWUgUENSIHByZXNlbnRhdGlvbiIgd2lkdGg9IjY0MCIgaGVpZ2h0PSIzNjAiIHNyYz0iaHR0cHM6Ly93d3cueW91dHViZS5jb20vZW1iZWQvRHdCSXZ6Z2dWQU0\/ZmVhdHVyZT1vZW1iZWQiIGZyYW1lYm9yZGVyPSIwIiBhbGxvdz0iYWNjZWxlcm9tZXRlcjsgYXV0b3BsYXk7IGNsaXBib2FyZC13cml0ZTsgZW5jcnlwdGVkLW1lZGlhOyBneXJvc2NvcGU7IHBpY3R1cmUtaW4tcGljdHVyZTsgd2ViLXNoYXJlIiByZWZlcnJlcnBvbGljeT0ic3RyaWN0LW9yaWdpbi13aGVuLWNyb3NzLW9yaWdpbiIgYWxsb3dmdWxsc2NyZWVuPjwvaWZyYW1lPg==\"><div class=\"brlbs-cmpnt-cb-preset-a\"> <p class=\"brlbs-cmpnt-cb-description\">You are currently viewing a placeholder content from <strong>Default<\/strong>. To access the actual content, click the button below. Please note that doing so will share data with third-party providers.<\/p> <div class=\"brlbs-cmpnt-cb-buttons\"> <a class=\"brlbs-cmpnt-cb-btn\" href=\"#\" data-borlabs-cookie-unblock role=\"button\">Unblock content<\/a> <a class=\"brlbs-cmpnt-cb-btn\" href=\"#\" data-borlabs-cookie-accept-service role=\"button\" style=\"display: none\">Accept required service and unblock content<\/a> <\/div> <a class=\"brlbs-cmpnt-cb-provider-toggle\" href=\"#\" data-borlabs-cookie-show-provider-information role=\"button\">More Information<\/a> <\/div><\/div>            <\/div>\n\n        <\/div>\n\n        <div class=\"column column--50\">\n            <h3>Explica\u00e7\u00e3o da PCR em Tempo Real<\/h3>\n            <p>O primeiro passo de toda an\u00e1lise \u00e9 a amostragem representativa e a homogeneiza\u00e7\u00e3o. Ap\u00f3s a lise das c\u00e9lulas org\u00e2nicas do tecido alimentar, o DNA \u00e9 preparado usando diferentes kits dedicados de prepara\u00e7\u00e3o de DNA ou RNA, espec\u00edficos para bact\u00e9rias ou v\u00edrus.<\/p>\n        <\/div>\n\n    \n<\/div>\n\n\n\n<div class=\"block-featuredvideo row\">\n\n    \n        <div class=\"column column--50\">\n\n            <div class=\"iframe-wrapper\">\n                 <div class=\"brlbs-cmpnt-container brlbs-cmpnt-content-blocker brlbs-cmpnt-with-individual-styles\" data-borlabs-cookie-content-blocker-id=\"default\" data-borlabs-cookie-content=\"PGlmcmFtZSB0aXRsZT0iVEFOQmVhZCBNYWVsc3Ryb23ihKIgOCBBdXRvc3RhZ2UgYW5kIHRoZSBTdXJlRmFzdMKuIE1hZyBQUkVQIEZvb2QiIHdpZHRoPSI2NDAiIGhlaWdodD0iMzYwIiBzcmM9Imh0dHBzOi8vd3d3LnlvdXR1YmUuY29tL2VtYmVkLzZCN2Q1UlZrRDVRP2ZlYXR1cmU9b2VtYmVkIiBmcmFtZWJvcmRlcj0iMCIgYWxsb3c9ImFjY2VsZXJvbWV0ZXI7IGF1dG9wbGF5OyBjbGlwYm9hcmQtd3JpdGU7IGVuY3J5cHRlZC1tZWRpYTsgZ3lyb3Njb3BlOyBwaWN0dXJlLWluLXBpY3R1cmU7IHdlYi1zaGFyZSIgcmVmZXJyZXJwb2xpY3k9InN0cmljdC1vcmlnaW4td2hlbi1jcm9zcy1vcmlnaW4iIGFsbG93ZnVsbHNjcmVlbj48L2lmcmFtZT4=\"><div class=\"brlbs-cmpnt-cb-preset-a\"> <p class=\"brlbs-cmpnt-cb-description\">You are currently viewing a placeholder content from <strong>Default<\/strong>. To access the actual content, click the button below. Please note that doing so will share data with third-party providers.<\/p> <div class=\"brlbs-cmpnt-cb-buttons\"> <a class=\"brlbs-cmpnt-cb-btn\" href=\"#\" data-borlabs-cookie-unblock role=\"button\">Unblock content<\/a> <a class=\"brlbs-cmpnt-cb-btn\" href=\"#\" data-borlabs-cookie-accept-service role=\"button\" style=\"display: none\">Accept required service and unblock content<\/a> <\/div> <a class=\"brlbs-cmpnt-cb-provider-toggle\" href=\"#\" data-borlabs-cookie-show-provider-information role=\"button\">More Information<\/a> <\/div><\/div>            <\/div>\n\n        <\/div>\n\n        <div class=\"column column--50\">\n            <h3>Seu caminho para a automa\u00e7\u00e3o<\/h3>\n            <p>Descubra a nova solu\u00e7\u00e3o walk-away: prepara\u00e7\u00e3o semi-automatizada de \u00e1cidos nucleicos para todas as amostras de alimentos e ra\u00e7\u00e3o com o <a href=\"https:\/\/food.r-biopharm.com\/products\/tanbead-maelstrom-8-autostage\">TANBead Maelstrom\u2122 8 Autostage<\/a> e o kit de extra\u00e7\u00e3o <a href=\"https:\/\/food.r-biopharm.com\/products\/surefast-autoprep-food\/\">SureFast\u00ae Mag PREP Food<\/a>.<\/p>\n        <\/div>\n\n    \n<\/div>\n\n\n\n<div class=\"block-featuredvideo row\">\n\n    \n        <div class=\"column column--50\">\n\n            <div class=\"iframe-wrapper\">\n                 <div class=\"brlbs-cmpnt-container brlbs-cmpnt-content-blocker brlbs-cmpnt-with-individual-styles\" data-borlabs-cookie-content-blocker-id=\"default\" data-borlabs-cookie-content=\"PGlmcmFtZSB0aXRsZT0iUklEQcKuQ1lDTEVSIOKAkyB0aGUgbXVsdGlwbGV4IHJlYWwtdGltZSBQQ1IgdGhlcm1vY3ljbGVyIiB3aWR0aD0iNjQwIiBoZWlnaHQ9IjM2MCIgc3JjPSJodHRwczovL3d3dy55b3V0dWJlLmNvbS9lbWJlZC9DX0NYLV9BemIwST9mZWF0dXJlPW9lbWJlZCIgZnJhbWVib3JkZXI9IjAiIGFsbG93PSJhY2NlbGVyb21ldGVyOyBhdXRvcGxheTsgY2xpcGJvYXJkLXdyaXRlOyBlbmNyeXB0ZWQtbWVkaWE7IGd5cm9zY29wZTsgcGljdHVyZS1pbi1waWN0dXJlOyB3ZWItc2hhcmUiIHJlZmVycmVycG9saWN5PSJzdHJpY3Qtb3JpZ2luLXdoZW4tY3Jvc3Mtb3JpZ2luIiBhbGxvd2Z1bGxzY3JlZW4+PC9pZnJhbWU+\"><div class=\"brlbs-cmpnt-cb-preset-a\"> <p class=\"brlbs-cmpnt-cb-description\">You are currently viewing a placeholder content from <strong>Default<\/strong>. To access the actual content, click the button below. Please note that doing so will share data with third-party providers.<\/p> <div class=\"brlbs-cmpnt-cb-buttons\"> <a class=\"brlbs-cmpnt-cb-btn\" href=\"#\" data-borlabs-cookie-unblock role=\"button\">Unblock content<\/a> <a class=\"brlbs-cmpnt-cb-btn\" href=\"#\" data-borlabs-cookie-accept-service role=\"button\" style=\"display: none\">Accept required service and unblock content<\/a> <\/div> <a class=\"brlbs-cmpnt-cb-provider-toggle\" href=\"#\" data-borlabs-cookie-show-provider-information role=\"button\">More Information<\/a> <\/div><\/div>            <\/div>\n\n        <\/div>\n\n        <div class=\"column column--50\">\n            <h3>O termociclador PCR em tempo real multiplex<\/h3>\n            <p><a href=\"https:\/\/food.r-biopharm.com\/products\/ridacycler\/\">RIDA\u00aeCYCLER<\/a> \u00e9 um termociclador PCR em tempo real compacto e f\u00e1cil de usar que permite uma an\u00e1lise r\u00e1pida e flex\u00edvel das suas amostras de alimentos e ra\u00e7\u00e3o. O software amig\u00e1vel permite iniciar rapidamente uma ou mais an\u00e1lises simultaneamente.<\/p>\n        <\/div>\n\n    \n<\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>SureFood\u00ae PREP Basic e SureFood\u00ae Prep Advanced kits<\/strong> s\u00e3o utilizados para alimentos s\u00f3lidos e ra\u00e7\u00f5es de origem vegetal ou de carne. Para bebidas, kits espec\u00edficos de prepara\u00e7\u00e3o de DNA ser\u00e3o usados dependendo da matriz, enquanto a nova linha QuickGEN permite um manuseio simples e direto.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">O sistema modular de prepara\u00e7\u00e3o e detec\u00e7\u00e3o de DNA pode ser usado de forma independente. A amplifica\u00e7\u00e3o do DNA alvo requer, al\u00e9m dos kits relevantes, o uso de um termociclador adequado com dois (FAM\/HEX) at\u00e9 quatro (FAM\/HEX\/ROX\/Cy5) canais de detec\u00e7\u00e3o.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>\u00c1reas de aplica\u00e7\u00e3o para PCR em testes alimentares<\/strong><br><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/food.r-biopharm.com\/wp-content\/uploads\/SureFood_Product_Overview_4p_EN_2023-06.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Vis\u00e3o Geral dos Produtos de PCR em Tempo Real<\/a><\/p>\n\n\n<div class=\"acf-block block-tabs block-\"><div class=\"block-tabs__menu\"><button onclick=\"openTab(event, 'tab--1',  'block-')\" class=\"block-tabs__title active \">Alerg\u00eanicos<\/button><button onclick=\"openTab(event, 'tab--2',  'block-')\" class=\"block-tabs__title  \">Especifica\u00e7\u00e3o Animal<\/button><button onclick=\"openTab(event, 'tab--3',  'block-')\" class=\"block-tabs__title  \">Contamina\u00e7\u00e3o de Bebidas<\/button><button onclick=\"openTab(event, 'tab--4',  'block-')\" class=\"block-tabs__title  \">GMO<\/button><button onclick=\"openTab(event, 'tab--5',  'block-')\" class=\"block-tabs__title  \">Microbiologia<\/button><\/div><div class=\"block-tabs__area\"><div class=\"block-tabs__content active tab--1\"><p>As alergias alimentares podem causar rea\u00e7\u00f5es imunol\u00f3gicas graves. Para proteger os consumidores, os produtos aliment\u00edcios devem ser testados para subst\u00e2ncias alerg\u00eanicas. Oferecemos uma ampla gama de kits de PCR para a detec\u00e7\u00e3o de al\u00e9rgenos como soja, avel\u00e3, amendoim, gl\u00faten, am\u00eandoa, aipo, gergelim, mostarda, peixe ou crust\u00e1ceos. Tamb\u00e9m oferecemos ensaios multiplex para a detec\u00e7\u00e3o simult\u00e2nea de m\u00faltiplos al\u00e9rgenos (amendoim\/avel\u00e3\/noz e aipo\/soja\/mostarda).<\/p>\n<\/div><div class=\"block-tabs__content  tab--2\"><p>A especifica\u00e7\u00e3o de esp\u00e9cies \u00e9 de grande interesse: produtos c\u00e1rneos podem ser falsificados devido \u00e0 declara\u00e7\u00e3o incorreta (veja o esc\u00e2ndalo da carne de cavalo). Outra aplica\u00e7\u00e3o \u00e9 a garantia de regras diet\u00e9ticas religiosas (halal, kosher). Nossa linha de produtos SureFood\u00ae Animal ID permite testes sens\u00edveis para porco, boi, cavalo, burro, frango, peru e outras esp\u00e9cies.<\/p>\n<\/div><div class=\"block-tabs__content  tab--3\"><p>Na produ\u00e7\u00e3o de bebidas, a detec\u00e7\u00e3o de organismos que causam deteriora\u00e7\u00e3o \u00e9 crucial. Com a linha de produtos GEN-IAL\u00ae, oferecemos uma ampla gama de kits de triagem para a detec\u00e7\u00e3o de organismos causadores de deteriora\u00e7\u00e3o na produ\u00e7\u00e3o de cerveja e vinho, como Lactobacillus, Pediococcus, Megasphaera, Pectinatus, Dekkera bruxellensis e Oenococcus oeni. Os kits de teste podem ser utilizados tanto para controle durante o processo quanto para controle do produto final.<\/p>\n<\/div><div class=\"block-tabs__content  tab--4\"><p>Em anos recentes, a produ\u00e7\u00e3o mundial de alimentos geneticamente modificados tem aumentado constantemente. Como os produtos aliment\u00edcios que cont\u00eam organismos geneticamente modificados (OGM) devem ser rotulados, a demanda por m\u00e9todos de teste sens\u00edveis est\u00e1 crescendo. Nossa linha de produtos SureFood\u00ae GMO permite a detec\u00e7\u00e3o qualitativa e quantitativa de OGM, como milho geneticamente modificado, arroz LibertyLink ou soja Roundup Ready.<\/p>\n<\/div><div class=\"block-tabs__content  tab--5\"><p>A detec\u00e7\u00e3o de contamina\u00e7\u00e3o microbiol\u00f3gica \u00e9 um dos principais campos de aplica\u00e7\u00e3o da PCR na an\u00e1lise de alimentos. Nossa linha de produtos SureFast\u00ae detecta uma ampla gama de bact\u00e9rias patog\u00eanicas (por exemplo, Salmonella, Listeria, Legionella, E. coli, Staphylococcus aureus, Campylobacter), v\u00edrus (por exemplo, Norov\u00edrus, Hepatite e Influenza) assim como leveduras e bact\u00e9rias de deteriora\u00e7\u00e3o.<\/p>\n<\/div><\/div><\/div>\n\n<\/div>\n\n\n\n<div class=\"wp-block-column column column--25 is-layout-flow wp-block-column-is-layout-flow\">\n\n<\/div>\n\n\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2273,"featured_media":0,"parent":340394,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"content-type":"","_relevanssi_hide_post":"","_relevanssi_hide_content":"","_relevanssi_pin_for_all":"","_relevanssi_pin_keywords":"","_relevanssi_unpin_keywords":"","_relevanssi_related_keywords":"","_relevanssi_related_include_ids":"","_relevanssi_related_exclude_ids":"","_relevanssi_related_no_append":"","_relevanssi_related_not_related":"","_relevanssi_related_posts":"","_relevanssi_noindex_reason":"","inline_featured_image":false,"footnotes":""},"class_list":["post-340839","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/food.r-biopharm.com\/pt-br\/wp-json\/wp\/v2\/pages\/340839","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/food.r-biopharm.com\/pt-br\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/food.r-biopharm.com\/pt-br\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/food.r-biopharm.com\/pt-br\/wp-json\/wp\/v2\/users\/2273"}],"replies":[{"embeddable":true,"href":"https:\/\/food.r-biopharm.com\/pt-br\/wp-json\/wp\/v2\/comments?post=340839"}],"version-history":[{"count":3,"href":"https:\/\/food.r-biopharm.com\/pt-br\/wp-json\/wp\/v2\/pages\/340839\/revisions"}],"predecessor-version":[{"id":347558,"href":"https:\/\/food.r-biopharm.com\/pt-br\/wp-json\/wp\/v2\/pages\/340839\/revisions\/347558"}],"up":[{"embeddable":true,"href":"https:\/\/food.r-biopharm.com\/pt-br\/wp-json\/wp\/v2\/pages\/340394"}],"wp:attachment":[{"href":"https:\/\/food.r-biopharm.com\/pt-br\/wp-json\/wp\/v2\/media?parent=340839"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}