Do you want to test beer for beverage spoilers? For fast and specific screening and detection of microbiological spoilage parameters in breweries, a wide product line of qPCR kits is offered.
Spoilage bacteria and yeasts can cause turbidity, change of taste and off-flavors in beers. To avoid losses or recalls, it is important to test for spoilage organisms in all areas of production. For fast and specific screening and detection of microbiological spoilage parameters in beer production, we offer a wide product line of test kits. The following spoilage bacteria and yeasts are of special interest in the beer industry and should be monitored:
Dekkera bruxellensis (anamorph Brettanomyces bruxellensis) is a spoilage yeast in beverages, especially important for the wine industry, where it has been shown to produce high amounts of volatile phenols (4-ethylphenol and 4-ethylguaiacol) imparting off-flavors to red wines. The yeasts of the species Dekkera bruxellensis are responsible for serious economic losses in the wine industry owing to their ability to spoil wines by the production of ethylphenols. Some authorities consider “brett” to be responsible for 90% of the spoilage problems in premium red wines. However, in the production of beer (especially craft beer or Belgian beer specialities), Dekkera bruxellensis is often not considered as contaminant, but as flavor enrichment.
Lactobacillus may be part of a regular fermentation process, but can also cause undesired acidification of beer or spoilage of fruit juices. Lactobacilli are killed by standard pasteurization.
Megasphaera are gram-negative, strictly anaerobic bacteria. They are well-known as beer spoilers producing off-flavors and turbidity.
Apart from Megaspaera, Pectinatus is another important spoilage bacterium in beer production. The gram-negative and strictly anaerobic bacterium causes turbidity and off-flavors reminiscent of rotten eggs.
Pediococcus is a genus of gram-positive lactic acid bacteria which are considered as contaminant in wine and beer. However, in some beer styles, the presence of Pediococcus may be desired.
Pichia is a genus of yeasts in the family Saccharomycetaceae. It is used in the fermentation process in winemaking, but it can spoil wine when present in high quantities. Pichia may also cause turbidity and off-flavors in beer.
Saccharomyces is a genus of fungi including various species of yeasts. One of the best known species is Saccharomyces cerevisiae which is used in the production of beer, wine or bread. The species Saccharomyces diastaticus is a spoilage yeast in the wine and beer industry.
Beer analysis with GEN-IAL® can help you to avoid product recalls, to minimize production losses and to prevent damage to brand image. We offer test systems for in-process control and/or for on-line final product control. Use different analytical modules to establish your customized monitoring of microbiological beer spoilage organisms in beer and beer products: Either extremely fast or maximal sensitive detection and specific identification is possible by combining different methods of sample preparation and detection with real-time PCR.
Rapid beer screening using QuickGEN & MyGo Pro
In this video, we show you how to use the real-time PCR cycler “MyGo Pro” for beer analysis. This is a very fast and comfortable way to test beer for various beer spoilage bacteria.
Our GEN-IAL® portfolio includes screening kits and beer-specific kits. The new GEN-IAL® QuickGEN line is characterized by a simple and rapid sampling and assay process, providing results within two hours after sampling. The test is based on filtration or centrifugation with subsequent qPCR and allows an extreme rapid and specific screening and detection in beer without pre-enrichment (reduced sensitivity, no differentiation in vitality). Analysis is very easy to perform even for untrained users.
The established qPCR kits based on pre-enrichment of the sample allow a sensitive screening and specific detection. The new concept of pre-coated 8-well qPCR strips (TPBD, TPYB) enables the user to screen and identify safely over 30 beer spoilage bacteria and yeasts in one qPCR run without misinterpretation and can be performed on common multiplex blockcyclers.
Test kits for beer analysis:
|GEN-IAL® QuickGEN First-Beer PCR Kit P1 und S. diastaticus Taqman® Screening – NEW||Qualitative detection of bacterial and S.diastaticus contaminations in filtrated beer and beer mixes.||QPP1SD 0048*|
|GEN-IAL® QuickGEN First-Beer Differentiation PCR Kit||Multiplex detection (30 species) and identification (19 species) of relevant beer spoilers (bacteria and yeasts)||QTPBD 0096*|
|GEN-IAL® QuickGEN First-Beer yeast and bacteria differentiation||Multiplex detection and identification of beverage spoiling bacteria and yeasts||QTPYB 0096*|
|GEN-IAL® QuickGEN First-Beer PCR Kit P1 Taqman® Screening||Qualitative detection of bacterial and yeast contaminations||QPP1T 0048*|
|GEN-IAL® QuickGEN First-Beer PCR Kit P1 Screening and hop resistance||DNA screening and differentiation of beer spoiling bacteria and hop resistance genes||QPP1HR 0050|
|GEN-IAL® QuickGEN P1 Screening and hop resistance TaqMan™||DNA screening and differentiation of beer spoiling bacteria and hop resistance genes||QPP1HR 0048*|
|GEN-IAL® QuickGEN First- Bacteria PCR Kit Biofilm||Specific DNA detection of Lactococcus lactis, Leuconostoc mesenteroides and Pichia anomala||QTPBF 0050|
|GEN-IAL® QuickGEN* hop resistance genes horA and horC / hitA and orf5 TaqMan™||Specific DNA detection of hop resistance genes||QTPHR 0050|
|GEN-IAL® Pectinatus spp./Megasphaera spp. TaqMan™||Specific DNA detection and differentiation of Pectinatus and Megasphaera||TPPMD 0050|
|GEN-IAL® First-Bacteria PCR Kit Enterobacteriacea||Multiplex PCR-detection of enterobacteriaceae as indicators for hygiene and postprocessing contaminations||TPENT 0050|
|GEN-IAL® QuickGEN First-Yeast PCR Kit Wild yeast||Differentiation of wild yeast||QTPWY 0096*|
|GEN-IAL® QuickGEN First-Yeast PCR Kit wild yeasts 1||Differentiation of wild yeast||QTPWY1 0050|
|GEN-IAL® QuickGEN First-Yeast PCR Kit wild yeasts 2||Differentiation of wild yeast||QTPWY 20050|
|GEN-IAL® QuickGEN First-Yeast PCR Kit Dekkera spp.||Specific DNA detection of Dekkera spp.||QTPYD 0050|
|GEN-IAL® QuickGEN Dekkera bruxellensis TaqMan™||Specific DNA detection of Dekkera bruxellensis||QTPYDB 0048*|
|GEN-IAL® QuickGEN First-Yeast PCR Kit Saccharomyces diastaticus||Detection of Saccharomyces diastaticus||QTPYSD 0048|
|GEN-IAL® Zygosaccharomyces bailii||Specific DNA detection of Zygosaccharomyces bailii||QTPYZB 0048low|
|GEN-IAL® QuickGEN First-Yeast differentiation||DNA screening and differentiation of yeasts||QYDIF 0096*|
|GEN-IAL® Dekkera anomala TaqMan™||Specific DNA detection of Dekkera anomala||TPYDA 0050|
|GEN-IAL® Pichia anomala TaqMan™||Specific DNA detection of Pichia anomala||TPYPA 0050|
|GEN-IAL® Saccharomyces diastaticus TaqMan™||Specific DNA detection of Saccharomyces diastaticus||TPYSD 0050|
|GEN-IAL® Pichia membranaefaciens TaqMan™||Specific DNA detection of Pichia membranaefaciens||TPYPM 0050|
|GEN-IAL® Bottom fermented yeast TaqMan™||Specific DNA detection of bottom fermented yeast||TPYUG 0050|
|GEN-IAL® Top fermented yeast TaqMan™||Specific DNA detection of top fermented yeast||TPYOG 0050|
|GEN-IAL® Dekkera bruxellensis TaqMan™||Specific DNA detection of Dekkera bruxellensis||TPYDB 0050 **|
|GEN-IAL® QuickGEN* Sample preparation filtration||DNA preparation of beverage samples, Filtration||FSE 0100|
|GEN-IAL® QuickGEN* Sample preparation centrifugation||DNA preparation of beverage samples, Centrifugation||CSE 0100|
|QuickGEN Yeast Sample Preparation Centrifugation||Microbial DNA-Extraction from samples with high yeast cell counts e.g. yeast tanks, propagation etc.||CSY0100|
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Questions? Tap into our team’s expertise. We’re here to support you and your business throughout the testing process to ensure your success