Do you want to test beer for beverage spoilers? For fast and specific screening and detection of microbiological spoilage parameters in breweries, a wide product line of qPCR kits is offered.

Spoilage bacteria and yeasts can cause turbidity, change of taste and off-flavors in beers. To avoid losses or recalls, it is important to test for spoilage organisms in all areas of production. For fast and specific screening and detection of microbiological spoilage parameters in beer production, we offer a wide product line of test kits. The following spoilage bacteria and yeasts are of special interest in the beer industry and should be monitored:

Brettanomyces (Dekkera)

Dekkera bruxellensis (anamorph Brettanomyces bruxellensis) is a spoilage yeast in beverages, especially important for the wine industry, where it has been shown to produce high amounts of volatile phenols (4-ethylphenol and 4-ethylguaiacol) imparting off-flavors to red wines. The yeasts of the species Dekkera bruxellensis are responsible for serious economic losses in the wine industry owing to their ability to spoil wines by the production of ethylphenols. Some authorities consider “brett” to be responsible for 90% of the spoilage problems in premium red wines. However, in the production of beer (especially craft beer or Belgian beer specialities), Dekkera bruxellensis is often not considered as contaminant, but as flavor enrichment.

Lactobacillus

Lactobacillus may be part of a regular fermentation process, but can also cause undesired acidification of beer or spoilage of fruit juices. Lactobacilli are killed by standard pasteurization.

Megasphaera

Megasphaera are gram-negative, strictly anaerobic bacteria. They are well-known as beer spoilers producing off-flavors and turbidity.

Pectinatus

Apart from Megaspaera, Pectinatus is another important spoilage bacterium in beer production. The gram-negative and strictly anaerobic bacterium causes turbidity and off-flavors reminiscent of rotten eggs.

Pediococcus

Pediococcus is a genus of gram-positive lactic acid bacteria which are considered as contaminant in wine and beer. However, in some beer styles, the presence of Pediococcus may be desired.

Pichia

Pichia is a genus of yeasts in the family Saccharomycetaceae. It is used in the fermentation process in winemaking, but it can spoil wine when present in high quantities. Pichia may also cause turbidity and off-flavors in beer.

Saccharomyces

Saccharomyces is a genus of fungi including various species of yeasts. One of the best known species is Saccharomyces cerevisiae which is used in the production of beer, wine or bread. The species Saccharomyces diastaticus is a spoilage yeast in the wine and beer industry.

Beer analysis can help you to avoid product recalls, to minimize production losses and to prevent damage to brand image. We offer test systems for in-process control and/or for on-line final product control. Use different analytical modules to establish your customized monitoring of microbiological beer spoilage organisms in beer and beer products: Either extremely fast or maximal sensitive detection and specific identification is possible by combining different methods of sample preparation and detection with real-time PCR.

Rapid beer screening using QuickGEN & MyGo Pro

In this video, we show you how to use the real-time PCR cycler “MyGo Pro” for beer analysis. This is a very fast and comfortable way to test beer for various beer spoilage bacteria.

Our portfolio includes QuickGen screening kits, GEN-IAL® screening kits and beer-specific kits. The new QuickGEN line is characterized by a simple and rapid sampling and assay process, providing results within two hours after sampling. The test is based on filtration or centrifugation with subsequent qPCR and allows an extreme rapid and specific screening and detection in beer without pre-enrichment (reduced sensitivity, no differentiation in vitality). Analysis is very easy to perform even for untrained users.

The established qPCR kits based on pre-enrichment of the sample allow a sensitive screening and specific detection. The new concept of pre-coated 8-well qPCR strips (TPBD, TPYB) enables the user to screen and identify safely over 30 beer spoilage bacteria and yeasts in one qPCR run without misinterpretation and can be performed on common multiplex blockcyclers.

GEN-IAL® product overview

You can find more information on our real-time PCR test kits for beer analysis in our free brochure.

Test kits for beer analysis:

Multiplex real-time PCR
ProductDescriptionArt. No.
GEN-IAL® QuickGEN First-Beer PCR Kit P1 und S. diastaticus Taqman® Screening – NEWQualitative detection of bacterial and S.diastaticus contaminations in filtrated beer and beer mixes.QPP1SD0048*
GEN-IAL® QuickGEN* First-Beer Differentiation PCR KitMultiplex detection (30 species) and identification (19 species) of relevant beer spoilers (bacteria and yeasts)QTPBD0096*
GEN-IAL® QuickGEN* First-Beer yeast and bacteria differentiationMultiplex detection and identification of beverage spoiling bacteria and yeastsQTPYB0096*
QuickGEN First-Beer PCR Kit P1 Taqman® ScreeningQualitative detection of bacterial and yeast contaminationsQPP1T0048*
GEN-IAL® QuickGEN First-Beer PCR Kit P1 Screening and hop resistanceDNA screening and differentiation of beer spoiling bacteria and hop resistance genesQPP1HR 0050
GEN-IAL® QuickGEN* P1 Screening and hop resistance TaqMan™DNA screening and differentiation of beer spoiling bacteria and hop resistance genesQPP1HR 0048*
GEN-IAL® QuickGEN First- Bacteria PCR Kit BiofilmSpecific DNA detection of Lactococcus lactis, Leuconostoc mesenteroides and Pichia anomalaQTPBF0050
Real-time PCR - bacteria
ProductDescriptionArt. No.
GEN-IAL® QuickGEN* hop resistance genes horA and horC / hitA and orf5 TaqMan™Specific DNA detection of hop resistance genesQTPHR 0050
GEN-IAL® Pectinatus spp./Megasphaera spp. TaqMan™Specific DNA detection and differentiation of Pectinatus and MegasphaeraTPPMD 0050
First-Bacteria PCR Kit EnterobacteriaceaMultiplex PCR-detection of enterobacteriaceae as indicators for hygiene and postprocessing contaminationsTPENT0050
Real-time PCR - yeasts
ProductDescriptionArt. No.
GEN-IAL® QuickGEN First-Yeast PCR Kit Wild yeastDifferentiation of wild yeastQTPWY0096*
GEN-IAL® QuickGEN First-Yeast PCR Kit wild yeasts 1Differentiation of wild yeastQTPWY10050
GEN-IAL® QuickGEN First-Yeast PCR Kit wild yeasts 2Differentiation of wild yeastQTPWY20050
GEN-IAL® QuickGEN First-Yeast PCR Kit Dekkera spp.Specific DNA detection of Dekkera spp.QTPYD 0050
GEN-IAL® QuickGEN Dekkera bruxellensis TaqMan™Specific DNA detection of Dekkera bruxellensisQTPYDB0048*
GEN-IAL® QuickGEN First-Yeast PCR Kit Saccharomyces diastaticusDetection of Saccharomyces diastaticusQTPYSD0048
GEN-IAL® Zygosaccharomyces bailiiSpecific DNA detection of Zygosaccharomyces bailiiQTPYZB0048low
GEN-IAL® QuickGEN First-Yeast differentiation PCR KitDNA screening and differentiation of yeastsQYDIF0096*
GEN-IAL® Dekkera anomala TaqMan™Specific DNA detection of Dekkera anomalaTPYDA 0050
GEN-IAL® Pichia anomala TaqMan™Specific DNA detection of Pichia anomalaTPYPA 0050
GEN-IAL® Saccharomyces diastaticus TaqMan™Specific DNA detection of Saccharomyces diastaticusTPYSD 0050
GEN-IAL® Pichia membranaefaciens TaqMan™Specific DNA detection of Pichia membranaefaciensTPYPM 0050
GEN-IAL® Bottom fermented yeast TaqMan™Specific DNA detection of bottom fermented yeastTPYUG 0050
GEN-IAL® Top fermented yeast TaqMan™Specific DNA detection of top fermented yeastTPYOG 0050
GEN-IAL® Dekkera bruxellensis TaqMan™Specific DNA detection of Dekkera bruxellensisTPYDB 0050 **

DNA preparation:

ProductDescriptionArt. No.
GEN-IAL® QuickGEN* Sample preparation filtrationDNA preparation of beverage samples, FiltrationFSE 0100
GEN-IAL® QuickGEN* Sample preparation centrifugationDNA preparation of beverage samples, CentrifugationCSE 0100
QuickGEN Yeast Sample Preparation CentrifugationMicrobial DNA-Extraction from samples with high yeast cell counts e.g. yeast tanks, propagation etc.CSY0100

What is the right test kit for you?

Our “decision tree” helps you to find the real-time PCR kit that best suits your needs in beer analysis.

Beverage spoilage support

Questions? Tap into our team’s expertise. We’re here to support you and your business throughout the testing process to ensure your success

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