SureFast® Fecal Screen 4plex
Art. No. F5504
Analytes
Product info about SureFast® Fecal Screen 4plex
Intended use
The SureFast® Fecal Screen 4plex is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific DNA sequences of Enterobacterales, Enterococci, Escherichia coli and Shigella spp. in water.
General Information
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The internal technical verification of instruments was performed on Agilent AriaDx, Bio-Rad CFX96, Bio-Rad CFX96 Dx, Qiagen Rotor-Gene Q, R-Biopharm RIDA®CYCLER and Roche LightCycler® 480 II.
| Specifications | |
|---|---|
| Art. No | F5504 |
| Test format | 100 reactions |
| Sample preparation | For DNA-preparation the use of SureFast® PREP Aqua (Art. Nr. F1023) is recommended. |
| LOD (Detection Limit) | The SureFast® Fecal Screen 4plex real-time PCR has a limit of detection of < 5 DNA copies. |
| Validated matrices | Water portions horse |
