SureFood® ALLERGEN Molluscs
Art. No. S3613
Product info about SureFood® ALLERGEN Molluscs
Intended use
The SureFood® ALLERGEN Molluscs is a real-time PCR for the direct, qualitative and / or quantitative detection of specific molluscs (mollusca) DNA sequences according to directive (EC) 1169/2011 in food.
The kit was developed for the identification of mussels (Bivalvia), cephalopods (Cephalopodae) and aquatic and terrestrial snails (Gastropodae). The molluscs family contains 50,000 – 60,000 species. Due to the large number of different species a practical verification of all is impossible. More detailed information is available in the Validation Report of SureFood® ALLERGEN Molluscs.
General Information
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical verification of instruments was performed on Agilent AriaDx, Bio-Rad CFX96 Dx, Qiagen Rotor-Gene Q, R-Biopharm RIDA®CYCLER, und Roche LightCycler® 480 II.
| Specifications | |
|---|---|
| Art. No | S3613 |
| Test format | 100 reactions |
| Sample preparation | For DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended. |
| LOD (Detection Limit) | The SureFood® ALLERGEN Molluscs PCR has a limit of detection of ≤ 0.4 mg / kg using SureFood® PREP Advanced, protocol 1. |
| LOQ (Limit of quantification | The limit of quantification is 1 mg / kg using SureFood® PREP Advanced, protocol 1. |