SureFood® ALLERGEN Molluscs
Art. No. S3613

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Product info about SureFood® ALLERGEN Molluscs

Intended use

The SureFood® ALLERGEN Molluscs is a real-time PCR for the direct, qualitative and / or quantitative detection of specific molluscs (mollusca) DNA sequences according to directive (EC) 1169/2011 in food.

The kit was developed for the identification of mussels (Bivalvia), cephalopods (Cephalopodae) and aquatic and terrestrial snails (Gastropodae). The molluscs family contains 50,000 – 60,000 species. Due to the large number of different species a practical verification of all is impossible. More detailed information is available in the Validation Report of SureFood® ALLERGEN Molluscs.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical verification of instruments was performed on Agilent AriaDx, Bio-Rad CFX96 Dx, Qiagen Rotor-Gene Q, R-Biopharm RIDA®CYCLER, und Roche LightCycler® 480 II.

Specifications
Art. NoS3613
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended.
LOD (Detection Limit)The SureFood® ALLERGEN Molluscs PCR has a limit of detection of ≤ 0.4 mg / kg using SureFood® PREP Advanced, protocol 1.
LOQ (Limit of quantification

The limit of quantification is 1 mg / kg using SureFood® PREP Advanced, protocol 1.