SureFood® QUANT SOFT WHEAT
Art. No. S7010
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Product info about SureFood® QUANT SOFT WHEAT
Intended use
SureFood® QUANT SOFT WHEAT is a real-time PCR kit for the detection of the relative soft wheat DNA content exclusively in durum wheat samples, such as pasta. The kit contains two PCR systems, one for detection of a wheat-specific gene (Triticum aestivum) and one for the detection of a wheat gene (Triticum spp., reference gene). The Positive Control corresponds to 3 % soft wheat in durum wheat.
General Information
The real-time PCR assay can be used with established real-time PCR instruments. The internal technical verification of instruments was performed on Roche LightCycler® 480 II, Bio-Rad CFX96 Dx, Agilent AriaDx, Bio-Rad CFX OPUS 96, R-Biopharm RIDA®CYCLER and Qiagen Rotor-Gene Q.
Zubehör
Dear customers,
we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st of January 2024, you can find our documents on the eIFU portal congen.de/information.
| Specifications | |
|---|---|
| Art. No | S7010 |
| Test format | 50 reactions |
| Sample preparation | For DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052), SureFood® PREP Advanced (Art. No. S1053) and SureFast® Mag PREP Food (Art. No. F1060) is recommended. |
| LOD (Detection Limit) | The SureFood® QUANT SOFT WHEAT specific PCR has a limit of detection of < 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content. |
| LOQ (Limit of quantification | The limit of quantification depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of the reference gene are present, the relative quantification limit for soft wheat DNA is 0.1 %. |
| Files | |
|---|---|
| Instructions | German/English |
