SureFast® Salmonella PLUS
Art. No. F5111
Product info about SureFast® Salmonella PLUS
Intended use
The SureFast® Salmonella PLUS is a real-time PCR for the direct, qualitative detection of a specific DNA sequence of Salmonella spp.. The SureFast® Salmonella PLUS real-time PCR kit has been validated and certified in combination with the SureFast® PREP Salmonella kit by AOAC (licence number: 041103).
Each reaction contains an internal amplification control (IAC).
General Information
The real-time PCR assay can be used with real-time PCR instruments, equipped for detection of two fluorescence emissions in channels FAM and VIC/HEX at the same time.
The technical validation of instruments was performed on Agilent AriaMx, Roche LightCycler® 480 II, Applied Biosystems® 7500, Bio-Rad CFX96 und Bio Molecular Systems MIC.
The internal technical verification of instruments (not part of the PTM validation) was performed on Roche LightCycler® 2.0, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, R-Biopharm RIDA®CYCLER, Cepheid SmartCycler, LTF MyGo Pro and Agilent Mx3005P.
| Specifications | |
|---|---|
| Art. No | F5111 |
| Test format | 100 reactions |
| Sample preparation | For DNA-preparation the use of SureFast® PREP Salmonella or SureFast® Speed PREP is recommended. |
| LOD (Detection Limit) | The SureFast® PATHOGEN Salmonella PLUS real-time PCR has a limit of detection of <5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content. |
