EuroProxima Plus Cheese Fraud
Art. No. 5171BKCC

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EuroProxima Plus Cheese Fraud

Art. No. 5171BKCC
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Intended use

EuroProxima Plus Cheese Fraud is a competitive enzyme immunoassay for screening of the presence of bovine milk in cheese of other species and sources.

General Information

Bovine Rennet Whey (BRW) is a by-product of cheese production from bovine milk. Adulteration of skimmed milk powder with BRW is cost attractive however illegal. Addition of BRW to dairy and other products can be identified by detecting the presence of the bovine glycomacropeptide (GMP), also named casein glycomacropeptide (CMP), which is released from bovine κ-casein (kappa-casein) by enzymatic (rennet) hydrolysis during cheese making process.
EuroProxima provides 3 ELISA kits for the detection of adulteration of food with bovine milk. The tests are based on monoclonal antibodies directed against small epitopes specific for bovine κ -casein protein. For fraud with cheese identity, a monoclonal antibody directed against a small epitope on the para-κ-casein part of the protein is used. The applied antibodies are specific for the detection of cow’s and buffalo’s milk. Moreover, the assays recognize denatured bovine-κ-casein in heat-treated and/or matured products due to the application of antibodies directed against only a small epitope in an inhibition assay format.

Accessories

Dear customers,

we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st of January 2024, you can find our documents on the eIFU portal eifu.r-biopharm.com/RBNL.

Specifications
Art. No5171BKCC
Test formatMicrotiter plate with 96 wells (12 strips with 8 wells each).
Sample preparationFor mentioned matrices fast and efficient extraction methods are included in the kit description.
Incubation time1 h 30 min (20 - 25 °C)
LOD (Detection Limit)1 % Cow's milk in cheese from other sources
Validated matrices

Cheese

Detected analyte

Para-κ-casein of glycomacropeptide.

EvaluationMicrotiter plate spectrophotometer (450 nm)
Files
InstructionsEnglish
MSDS5171bkcc.zip (English)