SureFood® 4plex LEGUMES
Art. No. S7008

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SureFood® 4plex LEGUMES

Art. No. S7008
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Intended use

The SureFood® 4plex LEGUMES is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific DNA sequences of legumes (Fabaceae), bean (Phaseolus spp. and Vigna spp.) and pea (Pisum sativum).

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical verification of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Bio-Rad CFX96 Dx, R-Biopharm RIDA®CYCLER and Agilent AriaDx.

Accessories

Dear customers,

we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st of January 2024, you can find our documents on the eIFU portal congen.de/information.

Specifications
Art. NoS7008
Test format100 reactions
Sample preparationFor DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052) and SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended.
LOD (Detection Limit)The SureFood® 4plex LEGUMES PCR has a limit of detection of <= 5 DNA-copies or 1 µg/mL (ppm) using SureFood® PREP Advanced, protocol 1.
The limit of detection and quantification of the complete method (DNA extraction and real-time PCR) depends on sample matrix, processing grade, DNA-preparation and DNA-content.