SureFood® 4plex LEGUMES
Art. No.: S7008
The SureFood® 4plex LEGUMES is a real-time PCR for the direct, qualitative detection and differentiation of specific DNA sequences of legumes (Fabaceae), bean (Phaseolus spp. and Vigna spp.) and pea (Pisum sativum).
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical verification of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Bio-Rad CFX96 Dx, R-Biopharm RIDA®CYCLER and Agilent AriaDx.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052) and SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended.|
|LOD (Detection Limit)||The SureFood® 4plex LEGUMES PCR has a limit of detection of <= 5 DNA-copies or 1 µg/mL (ppm) using SureFood® PREP Advanced, protocol 1.|
The limit of detection and quantification of the complete method (DNA extraction and real-time PCR) depends on sample matrix, processing grade, DNA-preparation and DNA-content.