SureFood® 4plex LEGUMES

Art. No.: S7008

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Product info

SureFood® 4plex LEGUMES

Art. No.: S7008

Intended use

The SureFood® 4plex LEGUMES is a real-time PCR for the direct, qualitative detection and differentiation of specific DNA sequences of legumes (Fabaceae), bean (Phaseolus spp. and Vigna spp.) and pea (Pisum sativum).

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical verification of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Bio-Rad CFX96 Dx, R-Biopharm RIDA®CYCLER and Agilent AriaDx.

Accessories

Dear customers,

we have started to provide the documents for our products in an electronic format. These are the Instructions for Use (IFU), the Safety Data Sheets (SDS) and the Certificate of Analysis (CoA). For batches placed on the market after 01 January 2023, you can find our documents on the eIFU portal eifu.r-biopharm.com.

Specifications
Art. NoS7008
Test format100 reactions
Sample preparationFor DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052) and SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended.
LOD (Detection Limit)The SureFood® 4plex LEGUMES PCR has a limit of detection of <= 5 DNA-copies or 1 µg/mL (ppm) using SureFood® PREP Advanced, protocol 1.
The limit of detection and quantification of the complete method (DNA extraction and real-time PCR) depends on sample matrix, processing grade, DNA-preparation and DNA-content.