SureFood® ALLERGEN Crustaceans
Art. No. S3612
Product info about SureFood® ALLERGEN Crustaceans
Intended use
The SureFood® ALLERGEN Crustaceans is a real-time PCR kit for the direct, qualitative and / or quantitative detection of specific crustaceans (Crustacea) DNA sequences according to directive (EC) 1169/2011. The crustacean family contains about 44,000 species. Due to the large number of different species a practical verification of all is impossible. More detailed information is available in the Validation Report.
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (Art. No. S3301) containing 40 mg crustaceans / kg food sample and a detection system including a standard series are required.
General Information
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent AriaDx, Bio-Rad CFX96 Dx, Bio-Rad CFX OPUS 96, Qiagen Rotor-Gene Q, R-Biopharm RIDA®CYCLER and Roche LightCycler® 480 II.
| Specifications | |
|---|---|
| Art. No | S3612 |
| Test format | 100 reactions |
| Sample preparation | For DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended. |
| LOD (Detection Limit) | The SureFood® ALLERGEN Crustaceans PCR has a limit of detection of ≤ 0.4 mg / kg using SureFood® PREP Advanced, protocol 1. |
| LOQ (Limit of quantification | 1 mg / kg using SureFood® PREP Advanced, protocol 1 |
