SureFood® 3plex APPLE/PEAR
Art. No. S7014

Product info about SureFood® 3plex APPLE/PEAR

SureFood® 3plex APPLE/PEAR

Art. No. S7014

Intended use

The SureFood® 3plex APPLE/PEAR is a multiplex real-time PCR kit for the direct, qualitative detection of specific DNA sequences of apple (Malus domestica) and pear (Pyrus communis) in food and feed.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX and Cy5 at the same time. The internal technical verification of instruments was performed on Roche LightCycler® 480 II, Roche LightCycler® PRO, Qiagen Rotor-Gene Q, Applied Biosystems™ QuantStudio™ 5, Bio-Rad CFX96 Dx, R-Biopharm RIDA®CYCLER and Agilent AriaDx.

Specifications
Art. No	S7014
Test format	100 reactions
Sample preparation	For DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended.
LOD (Detection limit)	The SureFood® 3plex APPLE/PEAR PCR has a limit of detection of  5 DNA copies or ≤ 4 mg / kg using SureFood® PREP Advanced, protocol 1.

SureFood® 3plex APPLE/PEAR Art. No. S7014