SureFood® ANIMAL ID 4plex LIVESTOCK Panel
Art. No. S6134
Analytes
Product info about SureFood® ANIMAL ID 4plex LIVESTOCK Panel
Intended use
The SureFood® ANIMAL ID 4plex LIVESTOCK Panel is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific
- chicken (Gallus gallus), turkey (Meleagris gallopavo), goose (Anser anser), muscovy duck (Cairina moschata), American pekin (Anas platyrhynchos),
- pork (Sus scrofa),
- camel (Camelus dromedarius),
- horse (Equus caballus),
- donkey (Equus asinus),
- water buffalo (Bubalis bubalis),
- beef (Bos taurus),
- sheep (Ovies aries)
- and goat (Capra hircus)
DNA sequences in food and feed.
General Information
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The internal technical verification of instruments was performed on Agilent AriaDx, Bio-Rad CFX96 Dx, R-Biopharm RIDA®CYCLER, Roche LightCycler® 480 II, Roche Lightcycler® PRO, Applied Biosystems QuantStudio 5 and Qiagen Rotor-Gene Q.
| Specifications | |
|---|---|
| Art. No | S6134 |
| Test format | 100 reactions |
| Sample preparation | For DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052), SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended. |
| LOD (Detection Limit) | The SureFood® ANIMAL ID 4plex LIVESTOCK Panel real-time PCR is developed for the detection of animal DNA in muscle meat mixture at a relative amount of 0.1 %. The limit of detection of the complete method (DNA extraction and real-time PCR) depends on sample matrix, processing grade, DNA-preparation and DNA-content. |
