EuroProxima AOZ

Art. No.: 5091AOZ

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EuroProxima AOZ

Art. No.: 5091AOZ
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Intended use

EuroProxima AOZ is a competitive enzyme immunoassay for quantitative analysis of AOZ in urine, tissue, milk, egg, honey and shrimps.

General Information

The nitrofurans are a group of synthetic broad-spectrum antibiotics, which have been widely and effectively used for the prevention and treatment of gastrointestinal infections caused by Escherichia coli, Salmonella spp., Mycoplasma spp., Coccidia spp., coliforms and other protozoa in animal production and aquaculture. Moreover, nitrofurans have been employed as growth promoters in livestock. The four major nitrofurans are furazolidone, furaltadone, nitrofurantoin and nitrofurazone. They have been banned in the EU for use as veterinary drugs, due to their toxic an suspected carcinogenic and mutagenic properties (Commission Regulation 1442/95). In 2003 a definitive MRPL (Minimum Required Performance Limit) was set at 1 ng/g (ppb) in the EU for all four of the above mentioned nitrofurans in poultry and aquaculture products (Commission Decision 2003/181/EC). Various studies have demonstrated that the nitrofuran parent molecules are rapidly metabolised by animals and that their in vivo stability is not longer than a few hours. As a result, persistent protein-bound residues are formed. Unlike the parent molecules, these protein-bound metabolites are stable and persistent in the body. It is possible to free these residues from proteins by acid hydrolysis. Testing for the presence of nitrofurans is thus equivalent to testing for the presence of a part of the parent molecule, i.e. the free residue. Nitrofuran residues are found after administration of furaltadone (3-amino-5-morpholinomethyl-2-oxazolidinone = AMOZ), furazolidone (3- amino-2-oxazolidinone = AOZ), nitrofurantoin (1-aminohydantoin = AHD) and nitrofurazone (semicarbazide = SEM).
Note: For the determination of AMOZ, AHD and SEM we refer to the EuroProxima ELISAs 5091AMOZ, 5091AHD and 5091SEM, respectively

Specifications
Art. No5091AOZ
Test formatMicrotiter plate with 96 wells (12 strips with 8 wells each).
Sample preparationFor mentioned matrices fast and efficient extraction methods are included in the kit description.
Incubation time45 min
LOD (Detection Limit)Urine (0.05 μg/L (ppb)), tissue (0.05 μg/kg), milk (0.05 μg/L), egg (0.05 μg/kg), honey (0.05 μg/kg), shrimps (0.05 μg/kg)
Validated matrices

Urine, tissue, milk, egg, honey and shrimps.

Detected analyte

AOZ in urine, tissue, milk, egg, honey and shrimps.

EvaluationMicrotiter plate spectrophotometer (450 nm)
Files
InstructionsEnglish
MSDS5091AOZ.zip (English)