Organismos modificados genéticamente

Soluciones innovadoras para la detección de GMO en los alimentos

  1. Home
  2. /
  3. Analitos
  4. /
  5. Organismos modificados genéticamente

Analitos

Organismos modificados genéticamente

La producción mundial de alimentos modificados genéticamente (por ejemplo, soja y maíz) ha aumentado de forma continua desde el comienzo de la década de los 90.

Desde el 1 de septiembre de 1998, todos los productos alimentarios que contienen organismos modificados genéticamente (GMO) se deben especificar en la etiqueta. De acuerdo con la normativa del Consejo de Ministros de la Unión Europea (UE), normativa para etiquetado de nuevos alimentos: N.º de decisión 1139/98/CE. Además, la directiva de la UE 1829/2003, que entró en vigor en abril de 2004, determina un valor umbral de etiquetado del 0,9 % en la UE para alimentos y piensos.

Detección cualitativa de GMO

De acuerdo con la normativa nacional o regional, se aplican unos niveles de tolerancia cero a los eventos de GMO no aprobados. De ahí que, por el momento, solo se consideren de interés los análisis cualitativos en estos productos. El límite de detección se encuentra en el 0,01 %, dependiendo de la matriz y la aplicación.

La regulación CE 619/2011 es una normativa especial para piensos. Siempre que se cumplan ciertas condiciones (caducidad de la aprobación de la UE, aprobación en el país de origen, disponibilidad de ensayos y material de referencia, etc.), se aplicará un valor umbral técnico del 0,1 % para eventos de GMO no aprobados. Esto es particularmente importante en las importaciones de maíz y soja con y sin GMO, no siempre libres de contaminación.

Detección cuantitativa de GMO

En los eventos de GMO aprobados respectivamente, existe la pregunta cuantitativa de si el producto supera los valores umbral definidos o los valores de etiquetado obligatorios.

En Europa, se aplica un valor del 0,9 % al evento de GMO en la matriz vegetal respectiva. Por debajo de este valor, no es necesario declarar productos alimentarios con contaminaciones casuales o técnicamente inevitables de eventos de GMO (CE 2829/2003 y EC2830/CE). La cuantificación se consigue a través de la cuantificación respectiva de un gen específico de GMO y un gen específico de matriz vegetal de acuerdo con una curva de calibración diferente en ambos casos, y la conversión al número de copias de ADN de GMO/matriz vegetal. Al multiplicar esta unidad adimensional por 100 se obtiene como resultado el valor de porcentaje. La curva de calibración se obtiene mediante la dilución de un estándar de ADN y, de acuerdo con un material de GMO definido, también se puede especificar de forma precisa con un factor de corrección experimental.

Cribado de GMO

El cribado de secuencias de genes extrínsecas a la planta infiltradas en ella durante el desarrollo del GMO proporciona una evidencia eficiente de la presencia de posibles GMO.

Las secuencias genéticas utilizadas habitualmente son las siguientes:

This promoter, made from the cauliflower mosaic virus, is the most frequently used element. Plant samples that are only 35S positive can also be contaminated with the naturally occurring virus. False positive results can be ruled out by using the virus-specific CaMV (S2027) test.

The Nopalin Synthase Terminator (NOS) from Agrobacterium tumefaciens is often but not exclusively used in combination with 35S.

In lieu of 35S, the figwort mosaic virus promoter can also be used in GMOs.

The gene for phosphinotricin-N-acetyltransferase from Streptomyces hygroscopicus can enable a resistance against the herbicide glufosinate that is available under several different brand names.

This sequence denotes the transition from chloroplast transit peptide to 5-enolpyruvylshikimate-3-phosphate synthase from Arabidopsis thaliana and Agrobacterium spp. It enables a resistance against the herbicide glyphosate.

Estos y otros parámetros se pueden combinar de forma modular o bien utilizar por separado. La eficiencia de la preparación del ADN se puede verificar mediante el kit PLANT Plus (S2049) específico de la planta.

SureFood® es un sistema modular basado en los 3 componentes principales, amplificación y detección. Cada componente consta de varios módulos. Todos los módulos son compatibles. Esto convierte a SureFood® es un sistema extremadamente flexible que se puede configurar fácilmente para diversas aplicaciones específicas del usuario. SureFood® está disponible como producto y también como servicio.

Resumen del producto SureFood® GMO

Encontrará un amplio resumen de los kits de ensayo SureFood® GMO en nuestro folleto gratuito.

Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
TANBead Maelstrom™ 4800

The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to …

Lee más
Up to 48 samples ZMAL48
SureFood® GMO QUANT MON810 Corn

The test detects the relative quantitative MON810 Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the MON810 Corn (OECD unique identifier MON-ØØ81Ø-6) and the other one specific for Corn (the reference gene). The …

Lee más
2 x 50 reactions S2019
SureFood® GMO ID DAS-40278-9 Corn

The SureFood® GMO ID DAS-40278-9 Corn is a real-time PCR kit for the direct, qualitative detection of a specific genetically modified corn DNA sequence. Therefore the kit contains an event specific real-time PCR system for the DAS-40278-9 corn (OECD …

Lee más
100 reactions S2140
SureFast® Mag PREP Food

The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 8 Autostage or Maelstrom 4800. Standardization and harmonization of …

Lee más
96 preparations F1060
TANBead Maelstrom™ 8 Autostage

The TANBead Maelstrom™ 8 Autostage (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing …

Lee más
Up to 8 samples ZMAL8
SureFood® GMO SCREEN 4plex BAR/PAT/CryIAb/IAc/ CTP2:CP4 EPSPS

The SureFood® GMO SCREEN 4plex BAR/PAT/CryIAb/IAc/CTP2:CP4 EPSPS is a real-time PCR for the direct, qualitative detection and differentiation of following specific genetically modified DNA – sequences. Phosphinothricin-Acetyltransferase gene …

Lee más
S2128
SureFood® GMO ID 4plex Corn I

The SureFood® GMO ID 4plex Corn I is a real-time PCR for the direct, qualitative detection and differentiation of following specific genetically modified corn DNA sequences:  FAM channel: MON810 corn (OECD unique identifier MON-ØØ81Ø-6) VIC/HEX …

Lee más
100 reactions S2170
SureFood® GMO ID 4plex Soya II

SureFood® GMO ID 4plex Soya II is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific genetically modified soya DNA sequences listed as below: Roundup Ready Soya (RR Soya) (OECD unique identifier …

Lee más
100 reactions S2162
SureFood® GMO ID 4plex Canola II

The multiplex test detects the following DNA-sequences of genetically modified canola: – FAM channel: MON88302 canola (OECD unique identifier MON-883Ø2-9) – VIC channel: Canola – ROX channel: DP73496 canola (OECD unique identifier …

Lee más
100 reactions S2167
SureFood® GMO ID 4plex Canola I

The multiplex test detects the following DNA sequences of genetically modified canola: FAM channel: MS8 canola (OECD unique identifier ACS-BNØØ5-8) ROX channel: GT73 canola (OECD unique identifier MON-ØØØ73-7) Cy5 channel: T45 canola (OECD …

Lee más
100 reactions S2166
SureFood® GMO ID 4plex Soya I

The multiplex test SureFood® GMO ID 4plex Soya I detects the following DNA-sequences of soya: FAM-channel: MON87708 Soja (OECD Bezeichnung MON-877Ø8-9) FAM-channel: CV127 Soja (OECD Bezeichnung BPS-CV127-9) VIC-channel: DP305423 Soja (OECD …

Lee más
100 reactions S2161
SureFood® GMO ID CV127 Soya (R&D Version)

Detection of CV 127 Soya.

Lee más
100 reactions S2066
SureFood® GMO ID DP305423 Soya (R&D Version)

The test detects DP305423 Soya (OECD unique identifier DP-3Ø5423-1) DNA. The event specific detection is according to the official method of the European Commission. The real-time PCR assay can be used with established real-time PCR instruments …

Lee más
100 reactions S2065
SureFood® GMO SCREEN 4plex BAR/NPTII/PAT/CTP2:CP4 EPSPS

This kit can be used for screening of genetically modified organisms (GMOs) in food, feed and seeds. The multiplex test detects the following DNA-sequences: Phosphinothricin-Acetyltransferase gene (BAR) from Streptomyces hygroscopius …

Lee más
100 reactions S2127
SureFood® GMO Plant 4plex Corn/Soya/Canola+IAC

The SureFood® GMO Plant 4plex Corn/Soya/Canola+IAC is a real-time PCR for the direct, qualitative detection and differentiation of following specific DNA sequences.

Lee más
100 reactions S2158
SureFood® GMO Plant 4plex Corn/Soya/Canola/Cotton

The SureFood® GMO Plant 4plex Corn/Soya/Canola/Cotton is a real-time PCR for the direct, qualitative detection and differentiation of following specific corn/soya/canola/cotton DNA sequences.

Lee más
100 reactions S2156
SureFood® GMO QUANT GT73 Canola

The test detects the relative quantitative GT73 Canola DNA amount. Therefore the kit contains two PCR systems, one specific for the GT73 Canola (OECD unique identifier MON-ØØØ73-7) and the other one specific for Brassicacea family (reference …

Lee más
2 x 50 reactions S2061
SureFood® GMO QUANT MON863 Corn

The test detects the relative quantitative MON863 Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the MON863 Corn (OECD unique identifier MON-ØØ863-5) and the other one specific for corn (the reference gene). The event …

Lee más
2 x 50 reactions S2051
SureFood® GMO ID A2704-12 Soya – Discontinued in 2021 – Replaced by S2161

The test detects A2704-12 Soya DNA. Therefore the kit contains an event specific real-time PCR system for the A2704-12 Soya (OECD unique identifier ACS-GMØØ5-3). The event specific detection is according to the official method of the European …

Lee más
100 reactions S2057
SureFood® GMO PLANT PLUS

The test detects DNA of plants qualitatively. Each reaction contains an internal amplification control (IAC).    

Lee más
100 reactions S2049
SureFood® GMO QUANT MIR162 Corn

The test detects the relative quantitative MIR162-Corn DNA amount. The kit contains two PCR systems, one event-specific for the MIR162-Corn (OECD unique identifier SYN-IR162-4) and the other one specific for Corn (reference system).  

Lee más
2 x 50 Reactions S2135
SureFood® GMO SCREEN 4plex 35S/NOS/FMV/IAC

This kit can be used for screening of genetically modified organisms (GMOs) in food, feed and seeds. For this purpose, PCR systems for detection of the 35S Cauliflower Mosaic Virus (CaMV) promoter DNA sequence, A. tumefaciens NOS terminator DNA …

Lee más
100 reactions S2126
SureFood® GMO SCREEN CaMV

The test detects CaMV DNA. The presence of CaMV DNA indicates an infestation with the cauliflower mosaic virus. Host of the CaMV are Brassicaceae plants. With the virus specific detection system SureFood® GMO CaMV it is possible to verify the …

Lee más
100 reactions S2027
SureFood® GMO QUANT T25 Corn

The test detects the relative quantitative T25-Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the T25-Corn (OECD unique identifier ACS-ZMØØ3-2) and the other one specific for Corn (reference system). The …

Lee más
100 reactions S2017
SureFood® GMO QUANT RR2Y Soya

The test detects the relative quantitative RoundupReady2 Yield-Soya DNA amount (RR2Yield-Soya). Therefore the kit contains two PCR systems, one specific for the RR2Yield-Soya (OECD unique identifier MON-89788-1) and the other one specific for Soya …

Lee más
2×50 reactions S2029
SureFood® GMO QUANT RoundUp Ready Soya

The test detects the relative quantitative Roundup Ready Soya DNA amount (RR-Soya). Therefore the kit contains two PCR systems, one specific for the Roundup Ready Soya (OECD unique identifier MON-Ø4Ø32-6) and the other one specific for Soya …

Lee más
2×50 reactions S2014
SureFood® GMO QUANT Bt176 Corn

The test detects the relative quantitative Bt176-Corn DNA amount. Therefore the kit contains two PCR systems, one construct specific for the Bt176-Corn (OECD unique identifier SYN-EV176-9) and the other one specific for Corn (reference system). …

Lee más
100 reactions S2015
SureFood® GMO QUANT Bt11 Corn

The test detects the relative quantitative Bt11 Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the event Bt11 Corn (OECD unique identifier SYN-BTØ11-1) and the other one specific for Corn (the reference gene). The …

Lee más
2 x 50 reactions S2016
SureFood® GMO QUANT 35S Soya

The test is developed for the relative estimation of 35S CaMV promoter DNA amount in only Soya containing products. Therefore the kit contains two PCR systems, one specific for the 35S CaMV promoter sequence and the other one specific for Soya (the …

Lee más
2×50 reactions S2028
SureFood® GMO QUANT 35S Corn

The test is developed for the relative estimation of the 35S CaMV DNA amount in only Corn containing products. Therefore the kit contains two PCR systems, one specific for the 35S CaMV promoter sequence and the other one specific for Corn (the …

Lee más
2×50 reactions, whereof 50 reactions are used for the quantification of the reference gene. S2020
SureFood® GMO SCREEN P35S:BAR Rice

The test detects the genetically construct P35S:BAR. The LibertyLink 601 (LL601 – OECD unique identifier BCS-OSØØ3-7) Rice and the LibertyLink 62 (LL62 – OECD unique identifier ACS-OSØØ2-5) Rice contains this genetically construct but …

Lee más
100 reactions, whereas 50 reactions are used for detection of the reference gene. S2022
SureFood® GMO ID Bt63 Rice

The test detects Bt63 Rice DNA. The detection of Bt63 Rice is a construct-specific real-time PCR assay. Additionally the SureFood® GMO ID Bt63 Rice kit contains a reference-PCR system for the detection of rice. The real time PCR system used in the …

Lee más
100 reactions S2024
load more